Because the disruption of circadian clock gene is a risk factor in many diseases such as obesity and cancer, it is important to monitor and analyzed the expression of the rhythm of the clock gene throughout the body over a long period of time. Although we previously reported on a new gene expression analysis system tracking a target position on the body surface of freely moving mice, the experimental apparatus required a large space. We have therefore developed an in vivo recording system using a portable photomultiplier tube (PMT) system attached to an optical fibre. Directly connecting the target area with the device, we could easily measure the photon counts in a very small space. However, little information is known about the characteristics of optical fibres when exposed to twisting/looping in association with a moving mouse and the effect of the surface of optical fibre. In the present study, we report on the characteristics of optical fibres to detect gene expression rhythm in freely moving mice. Using this portable optical device directly connected with a target area, we were able to measure the circadian rhythm of clock gene expression over a prolonged period in freely moving mice in a small space.

中文翻译：

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使用便携式光纤设备在自由移动条件下从嗅球记录小鼠period1基因表达。

由于昼夜节律时钟基因的破坏是许多疾病（例如肥胖症和癌症）的危险因素，因此在很长一段时间内监测和分析整个身体中时钟基因节律的表达很重要。尽管我们先前曾报道过一种新的基因表达分析系统，该系统跟踪自由移动的小鼠在体表上的目标位置，但实验装置需要很大的空间。因此，我们开发了一种体内记录系统，该系统使用连接到光纤的便携式光电倍增管（PMT）系统。直接将目标区域与设备相连，我们可以轻松地在很小的空间中测量光子数。但是，关于与移动的鼠标相关联的扭曲/成环时的光纤特性以及光纤表面的影响的信息知之甚少。在本研究中，我们报告了光纤的特征，以检测自由移动的小鼠中的基因表达节奏。使用这种与目标区域直接相连的便携式光学设备，我们能够在小空间中自由移动的小鼠中长时间测量时钟基因表达的昼夜节律。