Understanding the biomolecular interactions in a specific organelle has been a long‐standing challenge because it requires super‐resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super‐resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H₂O₂), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H₂O₂ level leads to disassociation of nucleic acid–protein complexes.

中文翻译：

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多响应荧光探针可通过超分辨率成像显示具有活性氧物种调节作用的线粒体核蛋白动力学。

了解特定细胞器中的生物分子相互作用是一项长期的挑战，因为它需要超分辨率成像才能解析多个生物大分子的空间位置和动态相互作用。两个关键的困难是用于超分辨率纳米显微镜的合适探针的缺乏和使用多个探针引起的复杂性。在此，我们报告了一种喹啉衍生物探针，该探针选择性地富集于线粒体，并响应过氧化氢（H ₂ O ₂），蛋白质和核酸，从而使线粒体核蛋白动力学可视化。STED纳米显微镜检查发现，蛋白质位于线粒体的ista部，并与核酸大量融合形成核蛋白，而H ₂ O ₂含量升高则导致核酸-蛋白质复合物解离。