The objective of this study is to present and critically analyze the current state of knowledge on the chromatographic analysis of chemical warfare agents (CWAs) and their metabolites in biological and biomedical samples originating from humans and animals. Fast and reliable analysis of these chemicals is extremely important at a time, when more and more cases of CWA intoxication take place by terrorist groups and radical non-state organizations use or plan to use chemical weapons to achieve their own psychological, political and military goals. The review builds on original scientific publications and reports published from 2010 onwards, including some valuable papers released before that year. The authors stated that during last ten years increased number of laboratories are focused not only on trace analysis of CWA (mostly nerve and blister agents) in environmental and biological samples, but the growing number of research are devoted to instrumental analysis of metabolites, adducts and degradation products of these substances. The identification of very low-level concentrations of CWA's metabolites and adducts in biological material is often more important and difficult than the identification of original CWA, because of lower level of concentration and a very large number of compounds are present in biological samples. Many of them are hydrolysis products, metabolites and adducts, and are present in the samples in the ionic and hydrophilic form. For this reason, different chromatographic methods, in most cases liquid chromatography, are used to perform an unambiguous analysis of biological samples: blood, urine, plasma, serum, tissues, hairs.

这项研究的目的是介绍和批判性地分析有关来自人类和动物的生物和生物医学样品中化学战剂（CWA）及其代谢物的色谱分析的当前知识水平。同时，越来越多的恐怖组织和激进的非国家组织使用或计划使用化学武器实现自己的心理，政治和军事目标，对这些化学物质进行快速可靠的分析非常重要。 。该综述基于从2010年开始发表的原始科学出版物和报告，包括该年之前发表的一些有价值的论文。作者指出，在过去十年中，越来越多的实验室不仅致力于环境和生物样品中CWA（主要是神经和起泡剂）的痕量分析，而且越来越多的研究致力于代谢产物，加合物和这些物质的降解产物。在生物材料中，非常低浓度的CWA代谢物和加合物的鉴定通常比原始CWA的鉴定更为重要和困难，因为较低的浓度水平和生物样品中存在大量的化合物。它们中的许多是水解产物，代谢产物和加合物，并且以离子和亲水形式存在于样品中。因此，采用不同的色谱方法