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Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells.
Structure ( IF 5.7 ) Pub Date : 2020-06-23 , DOI: 10.1016/j.str.2020.05.013
Saikat Chakraborty 1 , Julia Mahamid 1 , Wolfgang Baumeister 1
Affiliation  

Microtubules (MTs) are the most rigid elements of the cytoskeleton with in vitro persistence lengths (Lp) in the range of 1–6 mm. In cellular environments, however, MTs often appear strongly curved. This has been attributed to the forces acting upon them in situ where they are embedded in composite networks of different cytoskeletal elements. Hitherto, the nanoscale organization of these networks has remained largely uncharacterized. Cryo-electron tomography (cryo-ET) allowed to visualize and analyze the in situ structure of cytoskeletal networks in pristinely preserved cellular environments and at high resolution. Here, we studied the molecular organization of MTs and their interactions with the composite cytoskeleton in frozen-hydrated HeLa and P19 cells at different cell-cycle stages. We describe modulation of MT curvature correlated with the surrounding molecular architecture, and show that nanoscale defects occur in curved MTs. The data presented here contribute to constructing realistic models of cytoskeletal biomechanics.



中文翻译:

冷冻电子断层扫描揭示细胞骨架的纳米级组织及其与细胞内微管曲率的关系。

微管 (MTs) 是细胞骨架中最坚硬的元素,其体外持久长度 (L p ) 范围为 1-6 毫米。然而,在蜂窝环境中,MTs 经常出现强烈弯曲。这归因于原位作用于它们的力,其中它们嵌入在不同细胞骨架元素的复合网络中。迄今为止,这些网络的纳米级组织在很大程度上仍未得到表征。冷冻电子断层扫描 (cryo-ET) 允许对原位进行可视化和分析在原始保存的细胞环境和高分辨率下细胞骨架网络的结构。在这里,我们研究了 MTs 的分子组织及其与不同细胞周期阶段冷冻水合 HeLa 和 P19 细胞中复合细胞骨架的相互作用。我们描述了与周围分子结构相关的 MT 曲率的调制,并表明纳米级缺陷发生在弯曲的 MT 中。此处提供的数据有助于构建真实的细胞骨架生物力学模型。

更新日期:2020-09-01
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