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Role of laccase gene in wheat NILs differing at QTL-Fhb1 for resistance against Fusarium head blight
Plant Science ( IF 5.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.plantsci.2020.110574
Nancy Soni 1 , Niranjan Hegde 1 , Achal Dhariwal 2 , Ajjamada C Kushalappa 1
Affiliation  

Fusarium head blight (FHB), caused mainly by Fusarium graminearum (Fg), is one of the most severe diseases of wheat. It affects grain yield and quality due to mycotoxin contamination, which is harmful for both human and livestock consumption. Cell wall lignification, following pathogen invasion, is one of the innate defense responses. Plant laccases are known to lignify the secondary cell walls. A metabolo-genomics study identified laccase as one of the candidate genes in QTL-Fhb1 of wheat NILs derived from Sumai 3*5/Thatcher cross. Based on phylogenetics, it was named as TaLAC4. Real-time qPCR revealed a strongly induced expression of TaLAC4 in NIL-R. The VIGS based transient silencing of TaLAC4 in NIL-R resulted in an increased susceptibility leading to Fg spread within the entire spike in 15dpi, contrasting to non-silenced where the infection was limited to inoculated spikelets. Histopathology revealed thickened cell walls, mainly due to G-lignin, in non-silenced NIL-R, relative to silenced, in conjunction with higher total lignin content. Metabolic profiling of TaLAC4 silenced NILs identified the accumulation of several precursor metabolites higher in abundances upstream TaLAC4. These results confirm that the resistance function of TaLAC4 in NIL-R is due to pathogen-induced lignification of secondary cell walls in the rachis.

中文翻译:

漆酶基因在不同 QTL-Fhb1 的小麦 NIL 中对镰刀菌抗性的作用

镰刀菌赤霉病(FHB)主要由禾谷镰刀菌(Fg)引起,是小麦最严重的病害之一。由于霉菌毒素污染,它会影响粮食产量和质量,这对人类和牲畜消费都是有害的。病原体入侵后的细胞壁木质化是先天防御反应之一。已知植物漆酶使次生细胞壁木质化。一项代谢基因组学研究确定漆酶是源自 Sumai 3*5/Thatcher 杂交的小麦 NIL 的 QTL-Fhb1 中的候选基因之一。基于系统发育学,它被命名为TaLAC4。实时 qPCR 显示 TaLAC4 在 NIL-R 中强烈诱导表达。NIL-R 中基于 VIGS 的 TaLAC4 瞬时沉默导致易感性增加,导致 Fg 在 15dpi 的整个峰值内扩散,与非沉默相比,感染仅限于接种的小穗。组织病理学显示细胞壁增厚,主要是由于 G-木质素,在非沉默的 NIL-R 中,相对于沉默,以及更高的总木质素含量。TaLAC4 沉默的 NIL 的代谢谱确定了 TaLAC4 上游丰度较高的几种前体代谢物的积累。这些结果证实了 NIL-R 中 TaLAC4 的抗性功能是由于病原体诱导的轴中次生细胞壁的木质化。TaLAC4 沉默的 NIL 的代谢谱确定了 TaLAC4 上游丰度较高的几种前体代谢物的积累。这些结果证实了 NIL-R 中 TaLAC4 的抗性功能是由于病原体诱导的轴中次生细胞壁的木质化。TaLAC4 沉默的 NIL 的代谢谱确定了 TaLAC4 上游丰度较高的几种前体代谢物的积累。这些结果证实了 NIL-R 中 TaLAC4 的抗性功能是由于病原体诱导的轴中次生细胞壁的木质化。
更新日期:2020-09-01
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