Abnormal structural and functional connectivity in the striatum during neurological disorders has been reported using functional magnetic resonance imaging (fMRI), although the effects of cell-type specific neuronal stimulation on fMRI and related behavioral alterations are not well understood. In this study, we combined DREADD technology with fMRI ("chemo-fMRI") to investigate alterations of spontaneous neuronal activity. These were induced by the unilateral activation of dopamine D1 receptor-expressing neurons (D1-neurons) in the mouse dorsal striatum (DS). After clozapine (CLZ) stimulation of the excitatory DREADD expressed in D1-neurons, the fractional amplitude of low frequency fluctuations (fALFF) increased bilaterally in the medial thalamus, nucleus accumbens and cortex. In addition, we found that the gamma-band of local field potentials was increased in the stimulated DS and cortex bilaterally. These results provide insights for better interpretation of cell type-specific activity changes in fMRI.

中文翻译：

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fMRI 在直接纹状体投射神经元单侧化学遗传激活后检测双侧脑网络激活

已经使用功能磁共振成像 (fMRI) 报告了神经系统疾病期间纹状体的异常结构和功能连接，尽管细胞类型特异性神经元刺激对 fMRI 和相关行为改变的影响尚不清楚。在这项研究中，我们将 DREADD 技术与 fMRI（“chemo-fMRI”）相结合，以研究自发神经元活动的改变。这些是由小鼠背侧纹状体 (DS) 中多巴胺 D1 受体表达神经元 (D1 神经元) 的单侧激活诱导的。在对 D1 神经元中表达的兴奋性 DREADD 进行氯氮平 (CLZ) 刺激后，内侧丘脑、伏隔核和皮质中的低频波动 (fALFF) 的分数幅度双侧增加。此外，我们发现局部场电位的伽马带在受刺激的 DS 和双侧皮质中增加。这些结果为更好地解释 fMRI 中细胞类型特异性活性变化提供了见解。