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Assessment of the miniaturized liquid Ames microplate format (MPF™) for a selection of the test items from the recommended list of genotoxic and non-genotoxic chemicals.
Mutation Research/Genetic Toxicology and Environmental Mutagenesis ( IF 1.9 ) Pub Date : 2020-06-23 , DOI: 10.1016/j.mrgentox.2020.503218
Dimitrios Spiliotopoulos 1 , Cécile Koelbert 1
Affiliation  

The Ames microplate format (MPF™) is a miniaturized version of the plate agar Ames tests that takes advantage of a liquid microplate approach in 384-well plates with a color change-based readout. This method, already compared to the Ames test in Petri dishes, is used to assess the genotoxic potential of a variety of test items, including (but not limited to) chemicals, environmental samples, and drug candidates.

61 chemicals were selected from the updated recommended lists of genotoxic and non-genotoxic chemicals for assessment of the performance of new or improved genotoxicity tests and tested in up to five bacterial strains. The agreement with the data from the scientific literature (over 90%) confirms the reliability of the Ames MPF™ as a cost-effective and 3R-compliant alternative to the regulatory Ames test that allows to predict and evaluate chemicals’ mutagenicity in a faster, less laborious and, if available, automatable manner.



中文翻译:

评估微型液体 Ames 微孔板格式 (MPF™) 以从基因毒性和非基因毒性化学品推荐列表中选择测试项目。

Ames 微孔板格式 (MPF™) 是平板琼脂 Ames 测试的小型化版本,它利用了 384 孔板中的液体微孔板方法,并具有基于颜色变化的读数。这种方法已经与培养皿中的 Ames 测试进行了比较,用于评估各种测试项目的遗传毒性潜力,包括(但不限于)化学品、环境样本和候选药物。

从更新后的基因毒性和非基因毒性化学品推荐清单中选择了 61 种化学品,用于评估新的或改进的基因毒性测试的性能,并在多达 5 种细菌菌株中进行了测试。与科学文献数据的一致性(超过 90%)证实了 Ames MPF™ 作为监管 Ames 测试的具有成本效益且符合 3R 的替代方案的可靠性,可以更快地预测和评估化学品的致突变性,不那么费力,如果有的话,可以自动化的方式。

更新日期:2020-07-17
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