Abstract In order to improve the mechanical properties and antibacterial properties of skin tissue engineering scaffolds, a microspheres-reinforced composite construct with controlled release property was designed. The silver-loaded chitosan (CS) microspheres (Ag@CMs) were prepared by emulsified crosslinking method. Ag@CMs were introduced into CS acetic acid solution and then Ag@CMs embedded porous CS scaffolds were fabricated by lyophilization method. The results show that Ag@CMs are uniformly distributed inside porous CS scaffolds and there is no significant effect on the structure of the pores. The Ag@CMs/CS composite scaffolds possess high porosity (66.03 ± 0.15) %. The compressive strength and modulus of the composite scaffold reach up to 0.47 ± 0.03 MPa and 3.95 ± 0.06 MPa, respectively, significantly higher than pure CS scaffold. The in vitro drug delivery tests and antibacterial activity tests illustrate that composite scaffolds have efficient antibacterial properties and excellent drug sustained-release performance. MTT assay indicates that Ag@CMs/CS porous scaffold can provide an interconnected porous structure and large surface area for mouse fibroblast cell (L929) adhesion and proliferation. These results suggest that Ag@CMs/CS porous scaffold is a promising candidate for skin tissue engineering.

中文翻译：

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用于皮肤组织工程的载银微球增强壳聚糖支架

摘要 为了提高皮肤组织工程支架的力学性能和抗菌性能，设计了一种具有控释性能的微球增强复合结构。采用乳化交联法制备载银壳聚糖（CS）微球（Ag@CMs）。将 Ag@CMs 引入 CS 醋酸溶液中，然后通过冻干法制备 Ag@CMs 嵌入的多孔 CS 支架。结果表明，Ag@CMs 均匀分布在多孔 CS 支架内，对孔结构没有显着影响。Ag@CMs/CS 复合支架具有高孔隙率 (66.03 ± 0.15)%。复合支架的抗压强度和模量分别达到 0.47±0.03 MPa 和 3.95±0.06 MPa，明显高于纯 CS 支架。体外给药试验和抗菌活性试验表明，复合支架具有高效的抗菌性能和优良的药物缓释性能。MTT 分析表明，Ag@CMs/CS 多孔支架可以为小鼠成纤维细胞 (L929) 的粘附和增殖提供相互连接的多孔结构和大表面积。这些结果表明 Ag@CMs/CS 多孔支架是皮肤组织工程的一个有前途的候选者。