当前位置: X-MOL 学术Environ. Pollut. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Antibiotic resistance genes, bacterial communities, and functions in constructed wetland-microbial fuel cells: Responses to the co-stresses of antibiotics and zinc.
Environmental Pollution ( IF 8.9 ) Pub Date : 2020-06-23 , DOI: 10.1016/j.envpol.2020.115084
Hua Li 1 , Han Xu 2 , Hai-Liang Song 3 , Yi Lu 4 , Xiao-Li Yang 2
Affiliation  

The effects of the continuous accumulation of Zinc (Zn) on the fate of antibiotic resistance genes (ARGs) in constructed wetland-microbial fuel cells (CW-MFCs) remain unclear. In this study, the impacts of Zn addition and a circuit mode on antibiotic removal, occurrence of ARGs, the bacterial community, and bacterial functions were investigated in three groups of CW-MFCs. The results showed that continuous Zn exposure enriched the target ARGs during the initial stage, while excessive Zn accumulation decreased antibiotic removal and the abundance of ARGs. A principal component analysis demonstrated that ARGs and the bacterial community distribution characteristics were significantly impacted by the mass accumulation of antibiotics and Zn, as well as the circuit mode. A redundancy analysis, partial least squares path modeling, and Procrustes analysis revealed that the accumulation of antibiotics and Zn, the composition of the bacterial community, the circuit mode, and the abundance of intI associated with horizontal gene transfer jointly contributed to the distributions of ARGs in the electrodes and effluent. Moreover, continuous exposure to Zn decreased the bacterial diversity and changed the composition and function of the bacterial community predicted using PICRUSt tool. The co-occurrence of ARGs, their potential hosts and bacterial functions were further revealed using a network analysis. A variation partition analysis also showed that the accumulation of target pollutants and the circuit mode had a significant impact on the bacterial community composition and functions. Therefore, the interaction among ARGs, the bacterial community, bacterial functions, and pollutant accumulations in the CW-MFC was complex. This study provides useful implications for the application of CW-MFCs for the treatment of wastewater contaminated with antibiotics and heavy metals.



中文翻译:

人工湿地微生物燃料电池中的抗生素抗性基因,细菌群落和功能:对抗生素和锌共同胁迫的反应。

锌(Zn)的持续积累对人工湿地微生物燃料电池(CW-MFCs)中抗生素抗性基因(ARGs)的命运的影响尚不清楚。在这项研究中,研究了三组CW-MFCs中锌添加和电路模式对抗生素去除,ARGs的发生,细菌群落和细菌功能的影响。结果表明,连续的锌暴露在初始阶段丰富了目标ARGs,而过多的锌积累降低了抗生素的去除和ARGs的丰度。主成分分析表明,ARGs和细菌群落分布特征受到抗生素和Zn的大量积累以及电路模式的显着影响。冗余分析,局部最小二乘路径建模,整体与水平基因转移相关联,共同促进了ARGs在电极和废水中的分布。此外,连续暴露于锌会降低细菌多样性,并改变使用PICRUSt工具预测的细菌群落的组成和功能。使用网络分析进一步揭示了ARG,其潜在宿主和细菌功能的共存。变异分区分析还表明,目标污染物的积累和电路模式对细菌群落的组成和功能有重大影响。因此,CW-MFC中ARG,细菌群落,细菌功能和污染物积累之间的相互作用非常复杂。

更新日期:2020-06-29
down
wechat
bug