Abstract Polyacrylonitrile nanofibrous membrane (PAN) was prepared by electrospinning technique and was directly converted to polyamine membrane by 50 % and 100 % (w/w) ethylenediamine (EDA) and hexamethylenediamine (HDA), respectively. The aminated PAN nanofiber membrane equipped with protein liquid chromatography were used to evaluate the dynamic binding capacity for C-phycocyanin (CPC) and allophycocyanin (APC) from disrupted Spirulina platensis under different operating conditions. Experimental results showed that the contaminating proteins were completely removed during the flow and wash procedures. However, a large amount of APC and CPC can be retained on the membranes. Consequently, the aminated PAN membrane may have a high potential for use in the separation of APC/CPC and TP from a complex algae feedstock. In this study, a highly effective capture of APC/CPC can be achieved in one-step membrane chromatography, which can be directly employed as an antibacterial and antioxidant nanofiber membranes.

中文翻译：

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氨基化聚丙烯腈纳米纤维膜评价C-藻蓝蛋白和别藻蓝蛋白在钝顶螺旋藻中的动态结合性能

摘要 采用静电纺丝技术制备聚丙烯腈纳米纤维膜（PAN），并分别以50%和100%（w/w）乙二胺（EDA）和六亚甲基二胺（HDA）直接转化为聚胺膜。使用配备蛋白质液相色谱的胺化PAN纳米纤维膜评估不同操作条件下破碎的钝顶螺旋藻中C-藻蓝蛋白（CPC）和别藻蓝蛋白（APC）的动态结合能力。实验结果表明，污染蛋白质在流动和洗涤过程中被完全去除。然而，大量的 APC 和 CPC 可以保留在膜上。因此，胺化 PAN 膜在从复杂的藻类原料中分离 APC/CPC 和 TP 方面具有很高的潜力。在这项研究中，