The unconventional yeast Yarrowia lipolytica is known as a producer of extracellular lipases. Here we overexpressed extracellular lipase (YlLip2) in yeast strain Y. lipolytica AJD ΔXΔA-Lip2 harboring the overexpression cassette of the YALI0A20350 gene under the strong hybrid promoter UAS1B₁₆-TEF. To maintain a high level of YlLip2 production, two extracellular proteases of Y. lipolytica, AEPp and AXPp, were deleted. The purified recombinant YlLip2 presented optimal catalytic activities at 37 °C and pH 8.0. The effect of two lipopeptide biosurfactants, i.e., amphisin produced by Pseudomonas fluorescens DSS73 and viscosinamide secreted by P. fluorescens DR54, on the conformation and activity of YlLip2 was evaluated using spectral methods, surface tension, and the enzyme activity assay. YlLip2 demonstrated high tolerance of the tested biosurfactants and had greater activity retention after incubation with both biosurfactants. Finally, we observed that intrinsic fluorescence intensity of YlLip2 decreased significantly with increasing lipopeptides concentration ranging from 2.5 to 60 μM. Our results showed that both biosurfactants improve enzymatic activity of YlLip2 and might suggest better interaction of the substrate with the active site. These favorable characteristics make YlLip2 a prospective additive in the pharmaceutical, food, cosmetic, and detergent industries.

非常规酵母解脂耶氏酵母被称为细胞外脂肪酶的生产者。在这里我们在酵母菌株解脂耶氏酵母AJDΔXΔA-Lip2中过表达细胞外脂肪酶（YlLip2），该菌株在强杂交启动子UAS1B ₁₆ -TEF下具有YALI0A20350基因的过表达盒。为了维持高水平的Y1Lip2产生，删除了解脂耶氏酵母的两种胞外蛋白酶AEPp和AXPp。纯化的重组Y1Lip2在37°C和pH 8.0下表现出最佳的催化活性。2个脂肽生物表面活性剂，即通过amphisin产生的效果，荧光假单胞菌DSS73和viscosinamide通过分泌荧光假单胞菌使用光谱方法，表面张力和酶活性测定评估DR54对Y1Lip2的构象和活性的影响。YlLip2表现出对测试的生物表面活性剂的高耐受性，并且与两种生物表面活性剂一起孵育后具有更大的活性保留。最后，我们观察到YlLip2的固有荧光强度随着脂肽浓度从2.5到60μM的增加而显着降低。我们的结果表明，两种生物表面活性剂均可提高YlLip2的酶促活性，并可能表明底物与活性位点之间的相互作用更好。这些有利的特性使YlLip2成为制药，食品，化妆品和洗涤剂行业的预期添加剂。