In this paper, silver nanoparticles (Ag-NPs) colloidal suspensions with different particle sizes of 5 to 40 nm were prepared. Dynamic light scattering (DLS) technique showed that the average hydrodynamic diameters of Ag-NPs were much larger than the particle diameters obtained using transmission electron microscopy and UV-Vis spectroscopy. The as-prepared Ag-NPs with different average hydrodynamic diameters were incorporated in cotton fabrics by the pad-dry-cure method. The silver content before and after washing cycles were determined by inductively coupled plasma spectrometry (ICP). The antibacterial properties of the fabrics after 0, 5 and 10 laundering cycles against both the Gram-negative bacterium of Escherichia coli (E. coli) and the Gram-positive bacterium of Staphylococcus aureus (S. aureus) were examined and a clear volcano trend was observed between the bacterial reduction rate (BR) and the hydrodynamic diameter of Ag-NPs loaded on the fibers. The cotton fabric treated by the Ag-NPs with the hydrodynamic diameter of 52 nm, exhibited the highest antibacterial activity (about 98 %) after 10 laundering cycles, while the other samples on either side of the volcano were less active. The cytotoxicity of the cotton fabrics treated with Ag-NPs was assayed on mouse embryonic fibroblast cells and evaluated by an MTT assay. The results showed that Ag-NPs were not toxic. Field emission scanning electron microscopy (FESEM) obtained from the cotton fabric before and after washing cycles demonstrated that the Ag-NPs had tight bonds with the surface of cotton fabric.

在本文中，制备了具有5至40 nm不同粒径的银纳米粒子（Ag-NPs）胶体悬浮液。动态光散射（DLS）技术表明，Ag-NPs的平均流体动力学直径远大于使用透射电子显微镜和UV-Vis光谱学获得的粒径。通过垫干固化法将制备的具有不同平均流体动力学直径的Ag-NPs掺入棉织物中。洗涤循环前后的银含量通过电感耦合等离子体光谱法（ICP）确定。织物的0,5和10针对洗涤两个循环的革兰氏阴性细菌后的抗菌性能大肠杆菌（大肠杆菌）和革兰氏阳性细菌金黄色葡萄球菌（金黄色葡萄球菌）进行了检查，并在细菌还原速率（BR）和纤维上负载的Ag-NPs的流体动力学直径之间观察到清晰的火山趋势。经过10个洗涤周期后，用水动力学直径为52 nm的Ag-NPs处理的棉织物具有最高的抗菌活性（约98％），而火山两侧的其他样品的活性均较低。用Ag-NPs处理的棉织物的细胞毒性在小鼠胚胎成纤维细胞上进行测定，并通过MTT测定进行评估。结果表明，Ag-NPs无毒。从棉织物在洗涤循环之前和之后获得的场发射扫描电子显微镜（FESEM）表明，Ag-NP与棉织物的表面紧密结合。