Phytopathogens causing mycotoxicoses in maize are a potential threat to grain quality and availability in many maize producing countries including South Africa. The use of natural biological agents for controlling maize fusariosis and many other such plant diseases, as opposed to the more traditional use of chemicals, is growing in popularity globally, as a greater emphasis gets placed on public health. In this study, nine Fusarium inhibiting isolates representing a subset of 200 native Pseudomonas isolates from the maize rhizosphere of 10 different farms in the North West Province of South Africa were further evaluated for their in vitro biocontrol potential. Although few of the isolates (PS1.1_, PS1.22_, PS2.2, PS6.4_, PS6.8_, PS7.2_, PS8.3, PS8.6, and PS9.1) had impressive in vitro biosuppressive effects against Fusarium graminearum and Fusarium culmorum, while also producing biosurfactants, only isolate HARBPS9.1 showed consistent antifungal capacity along with maize seed bioprotection capability. The candidate antagonist HARBPS9.1 was molecularly characterized as a Pseudomonas fulva strain and was found to harbor multiple functional biosynthetic gene clusters after FTIR, NMR and ESI-Q-TOF-MS metabolomics investigation. P. fulva HARBPS9.1 bioprotective mechanism is attributed to the possible presence of hydrogen cyanide, pyrrolnitrin, and rhamnolipids.

在包括南非在内的许多玉米生产国，引起玉米中真菌毒素的植物病原体可能对谷物质量和供应构成威胁。与更传统的使用化学品相反，使用天然生物制剂控制玉米镰刀菌病和许多其他此类植物病害，在全球范围内越来越流行，因为人们更加重视公共卫生。在这项研究中，从南非西北省的10个不同农场的玉米根际中，代表200种天然假单胞菌分离物的9种抑制镰刀菌分离株的体外生物防治潜力得到了进一步评估。尽管很少有分离株（PS1.1 _， PS1.22 _， PS2.2，PS6.4 _，PS6.8 _， PS7.2 _， PS8.3，PS8.6和PS9.1）对禾谷镰刀菌和枯萎镰刀菌具有令人印象深刻的体外生物抑制作用，同时还产生生物表面活性剂，仅分离的HARBPS9.1表现出一致的抗真菌​​能力以及玉米种子的生物保护能力。候选拮抗剂HARBPS9.1的分子特征为假单胞菌，在FTIR，NMR和ESI-Q-TOF-MS代谢组学研究后发现具有多功能生物合成基因簇。P. fulva HARBPS9.1的生物保护机制归因于可能存在氰化氢，吡咯硝酸和鼠李糖脂。