Nanostructured wound dressings produced by electrospinning biocompatible polymers possess great potential because they resemble the natural extracellular matrix and support cell adhesion, proliferation, and differentiation. This study seeks to fabricate mupirocin, keratin, and coenzyme Q10 (Co Q10)-loaded PVA electrospun scaffolds intended for wound healing application and to characterize their morphology, physical properties, antibacterial activity, and biocompatibility. Polyvinyl alcohol (PVA) (10% w/v), various concentrations of keratin/Co Q10 fibrous scaffolds (electrospun at a voltage of 50 kV, flow rate of 4 mL/h), and 2% mupirocin was designed and fabricated to activate keratinocytes in the wound bed, stimulate cell proliferation, and increase antimicrobial penetration. The diameters of the scaffolds were observed to be in the nanoparticulate range 2.11 ± 0.20 to 3.27 ± 0.10 nm. By 30 min, all the scaffolds had more than 50% of the cumulative concentration of mupirocin released with 51.06 ± 2.104% to 74.66 ± 1.72% of mupirocin released. At 1 h, 80% of the mupirocin in the scaffold was seen to have diffused out of the scaffold. Release of mupirocin was modulated; an initial burst release was followed by sustained release over 2 h. Electrospun keratin/Co Q10/PVA scaffold containing mupirocin showed excellent antimicrobial activity against all the clinical isolates of 2586, Staphylococcus aureus 2590, 2583, 2587, 2555. All the electrospun scaffolds showed higher cell viability values than the control at 48 and 72 h, with the optimized CoQ10 scaffold concentration being 0.05% w/w. Electrospun nanofibers combining the biocompatibility potential of PVA with the bioactive nature of keratin (0.01% w/w) and CoQ10 (0.5% w/w) and the antibacterial property of mupirocin as a new potential for proper wound care was successfully developed. The cell line studies on this electrospun scaffold (PKCM 3) showed their ability to support the growth of keratinocytes hence the potential of developed scaffolds as a wound dressing. In vivo studies to further investigate the applications of the electrospun keratin/Co Q10/PVA nanofibrous scaffold as a wound dressing is however required.

中文翻译：

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含有莫匹罗星作为潜在伤口敷料的静电纺丝角蛋白/辅酶Q10 /聚乙烯醇纳米纤维支架的开发

通过电纺生物相容性聚合物生产的纳米结构伤口敷料具有巨大的潜力，因为它们类似于天然的细胞外基质并支持细胞粘附，增殖和分化。这项研究旨在制造用于伤口愈合的莫匹罗星，角蛋白和辅酶Q10（辅酶Q10）负载的PVA电纺支架，并表征其形态，物理性质，抗菌活性和生物相容性。设计并制造了聚乙烯醇（PVA）（10％w / v），各种浓度的角蛋白/辅酶Q10纤维支架（在50 kV电压下静电纺丝，流速为4 mL / h）和2％莫匹罗星以活化伤口床中的角质形成细胞，刺激细胞增殖，并增加抗微生物剂的渗透。观察到支架的直径在2.11±0.20至3.27±0.10nm的纳米颗粒范围内。到30分钟时，所有支架的莫匹罗星累积浓度均超过50％，其中莫匹罗星的释放浓度为51.06±2.104％至74.66±1.72％。在1小时时，观察到支架中80％的莫匹罗星已经扩散出支架。莫匹罗星的释放被调节；最初的突释释放，然后持续释放超过2小时。含有莫匹罗星的静电纺丝角蛋白/ Co Q10 / PVA支架对2586，金黄色葡萄球菌2590、2583、2587、2555的所有临床分离株均显示出优异的抗菌活性。在48和72 h，所有静电纺丝支架均显示出比对照更高的细胞活力值，最佳的CoQ10支架浓度为0.05％w / w。成功地开发了将PVA的生物相容性与角蛋白（0.01％w / w）和CoQ10（0.5％w / w）的生物活性性质以及莫匹罗星的抗菌特性相结合的电纺纳米纤维，作为适当伤口护理的新潜力。在该电纺丝支架（PKCM 3）上进行的细胞系研究表明，它们能够支持角质形成细胞的生长，因此具有开发出的支架作为伤口敷料的潜力。然而，需要体内研究以进一步研究电纺角蛋白/ Co Q10 / PVA纳米纤维支架作为伤口敷料的应用。在该电纺丝支架（PKCM 3）上进行的细胞系研究表明，它们能够支持角质形成细胞的生长，因此具有开发出的支架作为伤口敷料的潜力。然而，需要体内研究以进一步研究电纺角蛋白/ Co Q10 / PVA纳米纤维支架作为伤口敷料的应用。在该电纺丝支架（PKCM 3）上进行的细胞系研究表明，它们能够支持角质形成细胞的生长，因此具有开发出的支架作为伤口敷料的潜力。然而，需要体内研究以进一步研究电纺角蛋白/ Co Q10 / PVA纳米纤维支架作为伤口敷料的应用。