As a major sugar crop, sugar beet (Beta vulgaris L.) plays an important role in both sugar industry and feed products. Soil, acts as the substrate for plant growth, provides not only nutrients to plants but also a habitat for soil microorganisms. High soil fertility and good micro-ecological environment are basic requirements for obtaining high-yield and high-sugar sugar beets. This study aimed at exploring the effects of continuous cropping of sugar beet on its endophytic, soil bacterial community structures, and diversity. Using high-throughput sequencing technology which is based on Illumina Hiseq 2500 platform, the seeds of sugar beet (sample S), non-continuous cropping sugar beet (sample Bn) with its rhizosphere soil (sample Sr), and planting soil (sample Sn), continuous cropping sugar beet (sample Bc) with its planting soil (sample Sc), were collected as research materials. The results showed that the bacterial communities and diversity in each sample exhibited different OTU richness; 67.9% and 63.8% of total endophytic OTUs from samples Bc and Bn shared with their planting soil samples Sc and Sn, while sharing 36.4% and 31.8% of total OTUs with their seed sample S. Pseudarthrobacter and Bacillus as the two major groups coexisted among all samples, and other shared groups belonged to Achromobacter, Sphingomonas, Novosphingobium, Terribacillus, Planococcus, Paracoccus, Nesterenkonia, Halomonas, and Nocardioides. Genera, including Pantoea, Pseudomonas, Stenotrophomonas, Weissella, Leuconostoc, and Acinetobacter, were detected in each sugar beet sample but not in their corresponding soil sample. In this study, the bacterial community structures and soil compositions have significantly changed before and after continuous cropping; however, the effects of continuous cropping on endophytic bacteria of sugar beet were not statistically significant. This study would provide a scientific basis and reference information for in-depth research on correlations between continuous cropping and micro-ecological environment of sugar beet plant.

中文翻译：

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甜菜（重茬的影响甜菜通过高通量测序L.）在其内生和土壤细菌群落

甜菜（Beta vulgaris L.）作为主要的糖料作物，在制糖业和饲料产品中均起着重要作用。土壤作为植物生长的基质，不仅为植物提供营养，还为土壤微生物提供栖息地。高土壤肥力和良好的微生态环境是获得高产高糖甜菜的基本要求。这项研究旨在探讨甜菜连续种植对其内生，土壤细菌群落结构和多样性的影响。使用基于Illumina Hiseq 2500平台的高通量测序技术，对甜菜的种子（样品S），不连续作物的甜菜（样品Bn）及其根际土壤（样品Sr）和种植土壤（样品Sn ），连续种植甜菜（样品Bc）及其种植土壤（样品Sc），被收集为研究材料。结果表明，每个样品的细菌群落和多样性表现出不同的OTU丰富度。样品Bc和Bn的内生OTU总量中分别有67.9％和63.8％与种植土壤样品Sc和Sn共享，而种子样品S. Pdeudarthrobacter和芽孢杆菌则占总OTU的36.4％和31.8％，这两个主要群体共存所有样品和其他共享组均属于无色杆菌，鞘氨醇单胞菌，新孢子菌，特异芽孢杆菌，平球菌，副球菌，雀巢菌属，卤单胞菌和诺卡氏菌。在每个甜菜样品中均检出了包括泛菌，假单胞菌，嗜麦芽单胞菌，魏氏菌，亮粘菌和不动杆菌的属，但在其相应的土壤样品中却未检出。在这个研究中，连作前后细菌群落结构和土壤组成发生了明显变化；然而，连续种植对甜菜内生细菌的影响没有统计学意义。本研究为甜菜连作与微生态环境的相关性深入研究提供了科学依据和参考信息。