A high-throughput screening approach for simultaneous analysis and quantification of the percent conversion of up to 48 reactions has been developed using a thin-layer chromatography (TLC) imaging method. As a test-bed reaction, we monitored 48 thiol conjugate additions to a Meldrum’s acid derivative (1) in parallel using TLC. The TLC elutions were imaged using a cell phone and a LEGO brick-constructed UV/vis light box. Further, a spotting device was constructed from LEGO bricks that allows simple transfer of the samples from a well-plate to the TLC plate. Using software that was developed to detect “blobs” and report their intensity, we were able to quantitatively determine the extent of completion of the 48 reactions with one analysis.

中文翻译：

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下一代 TLC：并行点样和成像的定量平台。

使用薄层色谱 (TLC) 成像方法开发了一种高通量筛选方法，用于同时分析和量化多达 48 个反应的转化百分比。作为试验台反应，我们使用 TLC 平行监测了 48 个硫醇缀合物添加到 Meldrum 酸衍生物 ( 1 )。使用手机和乐高积木结构的紫外/可见光箱对 TLC 洗脱液进行成像。此外，一个点样装置由乐高积木构成，可以将样品从孔板简单地转移到 TLC 板。使用专为检测“斑点”并报告其强度而开发的软件，我们能够通过一次分析定量确定 48 次反应的完成程度。