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Riboflavin-Mediated Photooxidation of Gold Nanoparticles and Its Effect on the Inactivation of Bacteria.
Langmuir ( IF 3.9 ) Pub Date : 2020-06-22 , DOI: 10.1021/acs.langmuir.0c01473
María Belén Rivas Aiello 1 , Fiorela Ghilini 1 , Joaquín E Martínez Porcel 1 , Lisandro Giovanetti 1 , Patricia L Schilardi 1 , Daniel O Mártire 1
Affiliation  

Photodynamic inactivation (PDI) of microorganisms, based on the ability of photosensitizers to produce reactive oxygen species (ROS) under adequate irradiation, emerges as a promising technique to face the increasing bacterial resistance to conventional antimicrobials. In this work, we analyze the combined action of Riboflavin (Rf) and pectin-coated gold nanoparticles (PecAuNP) on Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) as suitable PDI strategy. We demonstrate that gold ions can be generated upon Rf-photosensitized oxidation of PecAuNP. Transient absorption spectroscopy shows that the Rf cationic radical can accept an electron from the nanoparticles to yield Au(I) ions, which in aqueous medium is disproportionate to yield Au0 and Au(III). Microbiological assays showed that the presence of PecAuNP enhanced the antibacterial activity of photoirradiated Rf toward S. aureus and P. aeruginosa, in line with the well-known antibacterial activity of gold ions. Moreover, the irradiation of Rf solutions containing about 100 μM PecAuNP enabled the solutions to be bactericidal against both bacteria.

中文翻译:

核黄素介导的金纳米粒子的光氧化作用及其对细菌灭活的影响。

基于光敏剂在足够的辐射下产生活性氧(ROS)的能力,微生物的光动力灭活(PDI)成为一种有前途的技术,可以应对日益增长的细菌对常规抗菌剂的耐药性。在这项工作中,我们分析了核黄素(Rf)和果胶包被的金纳米颗粒(PecAuNP)对金黄色葡萄球菌S. aureus)和铜绿假单胞菌P. aeruginosa)的联合作用)作为合适的PDI策略。我们证明Rf光敏化的PecAuNP氧化后可以生成金离子。瞬态吸收光谱表明,Rf阳离子自由基可以接受纳米颗粒中的电子以产生Au(I)离子,在水性介质中该离子不成比例产生Au 0和Au(III)。微生物学分析表明,PecAuNP的存在增强了光辐照的Rf对金黄色葡萄球菌铜绿假单胞菌的抗菌活性,这与众所周知的金离子抗菌活性一致。此外,含有约100μMPecAuNP的Rf溶液的辐照使溶液能够对两种细菌杀菌。
更新日期:2020-07-21
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