Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. The protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Data are available via ProteomeXchange with identifier PXD019423.

中文翻译：

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对 COVID-19 患者漱口液样本中的 SARS-CoV-2 蛋白进行质谱鉴定。

质谱 (MS) 可以提供有价值的诊断数据，补充基因组信息，使我们能够增加目前对 SARS-CoV-2 病毒引起的 COVID-19 疾病的了解。我们开发了一种基于 MS 的简单方法，专门检测 COVID-19 患者漱口液样本中的 SARS-CoV-2 蛋白。该方案包括对含漱液中的蛋白质进行丙酮沉淀和胰蛋白酶消化，然后进行靶向 MS 分析。我们的方法鉴定了源自 SARS-CoV-2 核蛋白的独特肽。基于这些有希望的初步结果，现在可以开发更快的 MS 协议作为 COVID-19 患者的常规诊断工具。数据可通过 ProteomeXchange 获得，标识符为 PXD019423。