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Slow-Release and Nontoxic Pickering Emulsion Platform for Antimicrobial Peptide.
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2020-06-19 , DOI: 10.1021/acs.jafc.0c00874
Luyun Cai 1, 2 , Minjie Cao 3 , Joe Regenstein 4
Affiliation  

The resistance in microorganisms against many conventional antibiotics has become a serious global health problem. However, antibacterial drug delivery materials are still limited in toxicity, short efficacy and reducing inflammation. The novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were tested as promising platforms to control bacterial resistance development. The parasin I interacted or conjugated with lecithin or chitosan and formed nanoparticles encapsulated by Pickering emulsion. The protonation and deprotonation of amino groups in chitosan and parasin I resulted in nanoparticles in different aggregate states and changed emulsion stability. Moreover, the Pickering emulsion could induce severe bacterial agglomeration on both Gram-positive and Gram-negative bacteria than parasin I through the membrane disintegration mechanism. Furthermore, the Pickering emulsion could alleviate the cytotoxicity of human liver cells and hemolytic activity in rat blood cells. In combination with the lack of acute cytotoxicity in Kunming mice and milder, more effective anti-inflammatory effect in peritonitis demonstrated for these Pickering emulsions, especially chitosan peptide-embedded nanoparticles Pickering emulsion, a potential role in combating multidrug resistant bacteria in biomedical applications.

中文翻译:

抗菌肽的缓释无毒Pickering乳剂平台。

微生物对许多常规抗生素的耐药性已成为严重的全球健康问题。然而,抗菌药物递送材料仍然在毒性,功效短和减轻炎症方面受到限制。测试了由抗菌肽纳米颗粒稳定的新型天然Pickering乳液,作为控制细菌耐药性发展的有前途的平台。parasin I与卵磷脂或壳聚糖相互作用或缀合,形成被Pickering乳液包裹的纳米颗粒。壳聚糖和parasin I中氨基的质子化和去质子化导致纳米粒子处于不同的聚集状态并改变了乳液的稳定性。此外,通过膜崩解机制,Pickering乳剂与革兰氏阴性菌I相比,可以在革兰氏阳性菌和革兰氏阴性菌上引起严重的细菌团聚。此外,Pickering乳液可以减轻人肝细胞的细胞毒性和大鼠血细胞的溶血活性。对于这些Pickering乳剂,尤其是壳聚糖肽包埋的纳米粒子Pickering乳剂,昆明小鼠缺乏急性细胞毒性,并且在腹膜炎中表现出更温和,更有效的抗炎作用,这在生物医学应用中对抗多药耐药细菌具有潜在作用。
更新日期:2020-07-15
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