Abstract The aim of this study was to investigate the association between expressions of five periodontal pathogens (Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf), Treponema denticola (Td) and Prevotella intermedia (Pi) and the periodontal pocket depth (PD) in people suffering from periodontitis. A study of 1164 plaque samples from individuals with periodontitis were conducted by multiplex real-time quantitative fluorescence-polymerase chain reaction to evaluate the PD and loads of five periodontal pathogens. The total bacterial number did not differ significantly between people with different subtypes of periodontitis. However, expression of specific pathogens (Pg, Td, Tf, Pi) showed a significant increase with the development of periodontitis. Expression of Pg, Td and Tf showed close correlation between them as well as with PD. Analyses of receiver operating characteristic curves showed that expression of Pg, Td, Tf and Pi had good potential for grading periodontitis severity. We found a close correlation between the relative expression of Pg, Td and Tf and PD. Measuring expression of target bacteria in the oral cavity could facilitate the understanding of the role of bacteria in periodontitis. Taking the appropriate steps to eliminate these pathogens might aid the prevention of periodontitis development.

中文翻译：

---

牙周病原体定量分析与牙周袋深度的关系

Td 和 Tf 显示它们之间以及与 PD 密切相关。受试者工作特征曲线的分析表明，Pg、Td、Tf 和 Pi 的表达对牙周炎严重程度的分级具有良好的潜力。我们发现 Pg、Td 和 Tf 的相对表达与 PD 之间存在密切相关性。测量口腔中目标细菌的表达有助于理解细菌在牙周炎中的作用。采取适当措施消除这些病原体可能有助于预防牙周炎的发展。测量口腔中目标细菌的表达有助于理解细菌在牙周炎中的作用。采取适当措施消除这些病原体可能有助于预防牙周炎的发展。测量口腔中目标细菌的表达有助于理解细菌在牙周炎中的作用。采取适当措施消除这些病原体可能有助于预防牙周炎的发展。