ABSTRACT Taxonomic information for New Zealand mosquito species is predominantly morphological with very few molecular data available to date. In this study, the mitochondrial COI gene and nuclear ITS1 were amplified and sequenced from DNA templates representing 17 species; 15 previously known New Zealand species, a newly discovered undescribed Aedes species from the Chatham Islands and a recently eradicated invader, Ae. camptorhynchus. This paper provides DNA barcoding sequences for the entire known New Zealand mosquito fauna, the first for the majority of these species. Phylogenetic analysis of COI and ITS1 indicated that the endemic species are all genetically distinct from the exotic species examined including vector species of pathogens of human diseases. The genus Opifex is distant from the genus Aedes based on analysis of ITS1 sequences, and Ae. chathamicus is more closely related to species within Aedes than to the genus Opifex. Results show Culex asteliae to be a valid species but Cx. rotoruae is not necessarily so. The Aedes species appears to be closely related to Ae. subalbirostris. The introduced Cx. quinquefasciatus was clearly shown to be related to Cx. pipiens; however, the endemic Cx. pervigilans was not. No evidence of population variation based on geographic location was detected.

中文翻译：

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新西兰蚊种的分子系统发育分析

摘要 新西兰蚊子物种的分类信息主要是形态学信息，迄今为止可用的分子数据很少。在这项研究中，从代表 17 个物种的 DNA 模板中扩增并测序了线粒体 COI 基因和核 ITS1；15 种以前已知的新西兰物种，一种来自查塔姆群岛的新发现的未描述的伊蚊物种，以及最近根除的入侵者伊蚊。喜鹊。本文提供了整个已知新西兰蚊子动物群的 DNA 条形码序列，这是大多数这些物种的第一个。COI 和 ITS1 的系统发育分析表明，特有物种在遗传上都不同于外来物种，包括人类疾病病原体的载体物种。根据对 ITS1 序列的分析，Opifex 属与伊蚊属相距甚远，和 Ae。chathamicus 与伊蚊内的物种的关系比与 Opifex 属的关系更密切。结果显示 Culex asteliae 是一个有效的物种，但 Cx。rotoruae 不一定如此。伊蚊物种似乎与伊蚊密切相关。亚口吻。介绍的 Cx。quinquefasciatus 被清楚地证明与 Cx 相关。pipiens; 然而，地方性 Cx。pervigilans 不是。没有检测到基于地理位置的人口变异的证据。