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Semiquantitative Detection of Hydrogen-Associated or Hydrogen-Free Electron Transfer within Methanogenic Biofilm of Microbial Electrosynthesis.
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-08-18 , DOI: 10.1128/aem.01056-20
Weiwei Cai 1, 2 , Wenzong Liu 3 , Bo Wang 4 , Hong Yao 1 , Awoke Guadie 4, 5 , Aijie Wang 2, 4
Affiliation  

Hydrogen-entangled electron transfer has been verified as an important extracellular pathway of sharing reducing equivalents to regulate biofilm activities within a diversely anaerobic environment, especially in microbial electrosynthesis systems. However, with a lack of useful methods for in situ hydrogen detection in cathodic biofilms, the role of hydrogen involvement in electron transfer is still debatable. Here, a cathodic biofilm was constructed in CH4-produced microbial electrosynthesis reactors, in which the hydrogen evolution dynamic was analyzed to confirm the presence of hydrogen-associated electron transfer near the cathode within a micrometer scale. Fluorescent in situ hybridization images indicated that a colocalized community of archaea and bacteria developed within a 58.10-μm-thick biofilm at the cathode, suggesting that the hydrogen gradient detected by the microsensor was consumed by the collaboration of bacteria and archaea. Coupling of a microsensor and cyclic voltammetry test further provided semiquantitative results of the hydrogen-associated contribution to methane generation (around 21.20% ± 1.57% at a potential of −0.5 V to −0.69 V). This finding provides deep insight into the mechanism of electron transfer in biofilm on conductive materials.

中文翻译:

微生物电合成产甲烷生物膜中氢缔合或无氢电子转移的半定量检测。

氢纠缠电子转移已被证明是重要的细胞外途径,可在多种厌氧环境中,特别是在微生物电合成系统中,共享减少的当量来调节生物膜的活性。但是,由于缺乏在阴极生物膜中进行原位氢检测的有用方法,氢在电子转移中的作用仍是有争议的。在这里,在CH 4生产的微生物电合成反应器中构建了阴极生物膜,在其中分析了氢的释放动力学,以证实在微米级范围内在阴极附近存在氢相关的电子转移。荧光原位杂交图像表明,古细菌和细菌的共定位群落在阴极处58.10μm厚的生物膜内形成,这表明由微传感器检测到的氢梯度被细菌和古细菌的协作所消耗。微传感器与循环伏安法测试的耦合进一步提供了与氢气相关的甲烷生成贡献的半定量结果(在-0.5 V至-0.69 V的电势下约为21.20%±1.57%)。该发现提供了对导电材料上生物膜中电子转移机理的深入了解。
更新日期:2020-08-19
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