Catecholamine synthesized in the sympathoadrenal system, including sympathetic neurons and adrenal chromaffin cells, is vital for cardiovascular homeostasis. It has been reported that GATA2, a zinc finger transcription factor, is expressed in murine sympathoadrenal progenitor cells. However, a physiological role for GATA2 in adrenal chromaffin cells has not been established. In this study, we demonstrate that GATA2 is specifically expressed in adrenal chromaffin cells. We examined the consequences of Gata2 loss‐of‐function mutations, exploiting a Gata2 conditional knockout allele crossed to neural crest‐specific Wnt1‐Cre transgenic mice (Gata2 NC‐CKO). The vast majority of Gata2 NC‐CKO embryos died by embryonic day 14.5 (e14.5) and exhibited a decrease in catecholamine‐producing adrenal chromaffin cells, implying that a potential catecholamine defect might lead to the observed embryonic lethality. When intercrossed pregnant dams were fed with synthetic adrenaline analogs, the lethality of the Gata2 NC‐CKO embryos was partially rescued, indicating that placental transfer of the adrenaline analogs complements the lethal catecholamine deficiency in the Gata2 NC‐CKO embryos. These results demonstrate that GATA2 participates in the development of neuroendocrine adrenaline biosynthesis, which is essential for fetal survival.

中文翻译：

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GATA2在肾上腺嗜铬细胞中起作用。

在交感肾上腺系统中合成的儿茶酚胺，包括交感神经元和肾上腺嗜铬细胞，对于心血管稳态至关重要。据报道，锌指转录因子GATA2在鼠交感肾上腺祖细胞中表达。但是，尚未确定GATA2在肾上腺嗜铬细胞中的生理作用。在这项研究中，我们证明了GATA2在肾上腺嗜铬细胞中特异性表达。我们研究的结果GATA2丧失功能的突变，利用一个GATA2条件性敲除等位基因杂交波峰特定神经的Wnt1 Cre重组酶转基因小鼠（GATA2 NC-CKO）。绝大多数Gata2NC-CKO胚胎在胚胎第14.5天（e14.5）死亡，并显示出产生儿茶酚胺的肾上腺嗜铬细胞减少，这表明儿茶酚胺潜在的缺陷可能导致观察到的胚胎致死性。当交叉怀孕的母坝喂食合成的肾上腺素类似物时，Gata2 NC-CKO胚胎的致死性得以部分挽救，这表明肾上腺素类似物的胎盘转移补充了Gata2 NC-CKO胚胎中的儿茶酚胺致死性不足。这些结果表明，GATA2参与神经内分泌肾上腺素生物合成的发展，这对胎儿的生存至关重要。