Chikungunya fever is a mosquito‐borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) is a rapid and simple tool used for DNA‐based diagnosis of a variety of infectious diseases. In this study, we established an RT‐LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene that could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito‐borne viruses. The system also amplified the E1 genome in the serum of CHIKV‐infected mice with high sensitivity and specificity. Moreover, we established a dry RT‐LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV‐infected patient samples with high accuracy. Thus, the dry RT‐LAMP system has great potential to be applied as a novel CHIKV screening kit in endemic areas.

中文翻译：

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开发便携式逆转录环介导的等温扩增系统，以经济高效的方式检测基孔肯雅病毒的E1区。

基孔肯雅热是由蚊子引起的疾病，持续性关节痛。基孔肯雅病毒（CHIKV）的当前诊断依赖于常规的逆转录聚合酶链反应分析。逆转录环介导的等温扩增（RT-LAMP）是一种快速简单的工具，可用于基于DNA的多种传染病诊断。在这项研究中，我们建立了一个RT-LAMP系统，通过靶向包膜蛋白1（E1）基因识别CHIKV，该基因也可以检测8 PFU浓度的CHIKV，而不会错误地检测其他蚊媒病毒。该系统还以高灵敏度和特异性扩增了CHIKV感染小鼠血清中的E1基因组。此外，我们建立了干式RT‐LAMP系统，无需冷链即可运输，可以在感染CHIKV的患者样本中高精度检测出病毒基因组。因此，干式RT‐LAMP系统在流行地区具有作为新型CHIKV筛查试剂盒的巨大潜力。