Terpene glycosides (TGs) in the berry skins of Ecolly and Cabernet Gernischet (CG) grapes were profiled by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and compared to that of Muscat Hamburg (MH) grapes. The aroma glycosides in grape skins were extracted by methanol, purified using Amberlite XAD-2 resins, eluted in methanol/ethyl acetate, and analyzed by UPLC-Q-TOF-MS. MS/MS fragmentation and the TG database were used for identification, while semiquantification was achieved by the internal standard, octyl-β-D-glucopyranoside. Seven, nine, and thirteen TGs were identified in Ecolly, CG, and MH, respectively, whereas only four TGs were detected in all the varieties. Compared to MH, linalool-3-O-α-L-arabinofuranosyl-β-D-glucopyranoside was unique for Ecolly, and pyran linalool oxide-α-L-arabinofuranosyl-β-D-glucopyranoside was unique for CG. Furthermore, the total concentrations of TGs were 7.32, 3.50, and 81.27 mg/kg in Ecolly, CG, and MH, respectively. Diendiol I-α-D-apiofuranosyl-β-D-glucopyranoside, furanosyl-linalool oxide-7-O-α-L-arabinofuranosyl-β-D-glucopyranoside, and nerol-l-O-α-D-apiofuranosyl-β-D-glucopyranoside were most abundant in Ecolly, CG, and MH, respectively. PRACTICAL APPLICATION: Terpene glycosides (TGs) are important aroma precursors for wines and it is meaningful to reveal the profiles of TGs in grapes. By analyzing the differences of TGs among the Ecolly, Cabernet Gernischet (CG), and Muscat Hamburg grape varieties, it is expected to provide guidance for the study on the aroma characteristics and aroma enhancing brewing of Ecolly and CG grapes. The results may provide technical experience on aroma glycoside identification and their quantification in grapes and wines for future studies.

中文翻译：

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使用超高效液相色谱-四极杆飞行时间质谱法分析 ecolly、赤霞珠和马斯喀特汉堡葡萄中的萜糖苷

通过超高效液相色谱-四极杆飞行时间质谱 (UPLC-Q-TOF-MS) 分析 Ecolly 和 Cabernet Gernischet (CG) 葡萄浆果皮中的萜糖苷 (TG) 并与汉堡马斯喀特进行比较(MH) 葡萄。葡萄皮中的芳香苷用甲醇提取，用Amberlite XAD-2树脂纯化，用甲醇/乙酸乙酯洗脱，用UPLC-Q-TOF-MS分析。MS/MS 碎裂和 TG 数据库用于鉴定，而半定量则由内标辛基-β-D-吡喃葡萄糖苷实现。在 Ecolly、CG 和 MH 中分别鉴定出 7、9 和 13 种 TG，而在所有品种中仅检测到 4 种 TG。与 MH 相比，芳樟醇-3-O-α-L-阿拉伯呋喃糖基-β-D-吡喃葡萄糖苷是 Ecolly 独有的，和吡喃芳樟醇氧化物-α-L-阿拉伯呋喃糖基-β-D-吡喃葡萄糖苷是CG独有的。此外，在 Ecolly、CG 和 MH 中，TG 的总浓度分别为 7.32、3.50 和 81.27 mg/kg。Diendiol I-α-D-吡喃呋喃糖基-β-D-吡喃葡萄糖苷、呋喃糖基-芳樟醇氧化物-7-O-α-L-阿拉伯呋喃糖基-β-D-吡喃葡萄糖苷和橙花醇-10-α-D-吡喃呋喃糖苷-β-D -吡喃葡萄糖苷分别在 Ecolly、CG 和 MH 中含量最高。实际应用：萜烯糖苷（TGs）是葡萄酒重要的香气前体，揭示葡萄中的TGs具有重要意义。通过分析Ecolly、Cabernet Gernischet (CG)和Muscat Hamburg等葡萄品种TGs的差异，有望为Ecolly和CG葡萄的香气特征及增香酿造研究提供指导。