Biodegradable multi‐l‐arginyl‐poly‐l‐aspartate (MAPA), more commonly cyanophycin, prepared with recombinant Escherichia coli contains a polyaspartate backbone with lysine and arginine as side chains. Two assemblies of polyelectrolyte multilayers (PEMs) are fabricated at three different concentration ratios of insoluble MAPA (iMAPA) with hyaluronic acid (iMAPA/HA) and with γ‐polyglutamic acid (iMAPA/γ‐PGA), respectively, utilizing a layer‐by‐layer approach. Both films with iMAPA and its counterpart, HA or γ‐PGA, as the terminal layer are prepared to assess the effect on film roughness, cell growth, and cell migration. iMAPA incorporation is higher for a higher concentration of the anionic polymer due to better charge interaction. The iMAPA/HA films when compared to iMAPA/γ‐PGA multilayers show least roughness. The growth rates of L929 fibroblast cells on the PEMs are similar to those on glass substrate, with no supplementary effect of the terminal layer. However, the migration rates of L929 cells increase for all PEMs. γ‐PGA incorporated films impart 50% enhancement to the cell migration after 12 h of culture as compared to the untreated glass, and the smooth films containing HA display a maximum 82% improvement. The results present the use of iMAPA to construct a new layer‐by‐layer system of polyelectrolyte biopolymers with a potential application in wound dressing.

中文翻译：

---

透明质酸和γ-聚谷氨酸多1-精氨酰基-聚-1-天冬氨酸配对制备的聚电解质多层的伤口愈合特性。

可生物降解的多升-arginyl -聚-升-天冬氨酸（MAPA），更常见的藻青素，重组制备的大肠杆菌含有聚天冬氨酸骨架，其中赖氨酸和精氨酸为侧链。分别用透明质酸（iMAPA / HA）和γ-聚谷氨酸（iMAPA /γ-PGA）的三种不同浓度比的不溶性MAPA（iMAPA）制备了两个聚电解质多层（PEM）组件，层方法。两种均以iMAPA及其对应的HA或γ-PGA作为末端层的膜均准备评估对膜粗糙度，细胞生长和细胞迁移的影响。由于较高的电荷相互作用，对于较高浓度的阴离子聚合物，iMAPA的掺入量较高。与iMAPA /γ-PGA多层膜相比，iMAPA / HA膜的粗糙度最低。L929成纤维细胞在PEM上的生长速率与在玻璃基板上的相似，没有末端层的辅助作用。然而，对于所有PEM，L929细胞的迁移速率都会增加。与未处理的玻璃相比，掺有γ-PGA的薄膜在培养12小时后能使细胞迁移增强50％，而含HA的光滑薄膜最大可提高82％。结果表明，使用iMAPA可以构建新的聚电解质生物聚合物逐层体系，并可能在伤口敷料中应用。