Treatment for peripheral nerve injuries includes the use of autografts and nerve guide conduits (NGCs). However, outcomes are limited, and full recovery is rarely achieved. The use of nerve scaffolds as a platform to surface immobilize neurotrophic factors and deliver locally is a promising approach to support neurite and nerve outgrowth after injury. We report on a bioactive surface using functional amine groups, to which heparin binds electrostatically. X‐ray photoelectron spectroscopy analysis was used to characterize the presence of nitrogen and sulfur. Nerve growth factor (NGF) and brain‐derived neurotrophic factor (BDNF) were bound by electrostatic interaction to heparin, and the release profile evaluated by enzyme‐linked immunosorbent assay, which showed that ca. 1% of NGF was released from each of the bioactive surface within 7 days. Furthermore, each surface showed a maximum release of 97% of BDNF. Neurotrophin release on neurite outgrowth was evaluated by primary dorsal root ganglion with a maximum neurite growth response in vitro of 1,075 µm detected for surfaces immobilized with NGF at 1 ng/ml. In summary, the study reports on the design and construction of a biomimetic platform to deliver NGF and BDNF using physiologically low concentrations of neurotrophin. The platform is directly applicable and scalable for improving the regenerative ability of existing NGCs and scaffolds.

中文翻译：

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NGF 和 BDNF 的仿生表面递送以增强神经突生长。

周围神经损伤的治疗包括使用自体移植物和神经导管 (NGC)。然而，结果是有限的，很少能完全康复。使用神经支架作为平台来固定神经营养因子并局部递送是支持损伤后神经突和神经生长的有前途的方法。我们报告了使用功能性胺基团的生物活性表面，肝素以静电方式与之结合。X射线光电子能谱分析用于表征氮和硫的存在。神经生长因子 (NGF) 和脑源性神经营养因子 (BDNF) 通过静电相互作用与肝素结合，酶联免疫吸附试验评估释放曲线，结果显示约 在 7 天内从每个生物活性表面释放 1% 的 NGF。此外，每个表面都显示出 97% 的最大 BDNF 释放。神经营养因子对神经突生长的释放通过初级背根神经节评估，体外检测到的最大神经突生长反应为 1,075 µm，用于固定有 1 ng/ml 的 NGF 的表面。总之，该研究报告了仿生平台的设计和构建，以使用生理学低浓度的神经营养因子来递送 NGF 和 BDNF。该平台可直接适用和可扩展，用于提高现有 NGC 和支架的再生能力。该研究报告了仿生平台的设计和构建，以使用生理学低浓度的神经营养因子来输送 NGF 和 BDNF。该平台可直接适用和可扩展，用于提高现有 NGC 和支架的再生能力。该研究报告了仿生平台的设计和构建，以使用生理学低浓度的神经营养因子来输送 NGF 和 BDNF。该平台可直接适用和可扩展，用于提高现有 NGC 和支架的再生能力。