An 8‐week feeding trial was conducted to evaluate the effects of replacement of fish oil (FO) with blending vegetable oils (VOs) on growth performance, antioxidant enzyme activities and fatty acid composition in tissue of swimming crab Portunustrituberculatus. Five isonitrogenous and isolipidic diets were formulated to contain VOs (colza oil: palm oil: linseed oil = 4:2:1) to replace 0 (the control diet), 250, 500, 750 and 1000 g/kg of FO (defined D0, D25, D50, D75, D100). Three hundred juvenile swimming crabs (initial weight 2.34 ± 0.08 g) were randomly stocked and sorted into 300 individual rectangle plastic baskets in three cement pools. Each treatment has three replicates, one replicate has 20 swimming crabs, and each diet fed 60 crabs distributed in 60 baskets. The results indicated that crabs fed the control diet showed significantly higher survival, final body weight, per cent weight gain (PWG), specific growth rate and moulting frequency, crude protein and crude lipid contents in muscles than those fed the D75 and D100 VO diets (p < .05). Crabs fed the D25 VO diet showed significantly higher concentration of triglyceride, low‐density lipoprotein cholesterol and total protein, activities of superoxide dismutase, catalase and glutathione peroxidase (GSH‐Px) in haemolymph than those fed the control diet (p < .05). Fatty acid composition in hepatopancreas was positively correlated with dietary composition. In summary, based on the PWG, the optimal replacement of FO with VOs was estimated to be 250 g/kg. These findings demonstrated that swimming crabs make better use of FO than VOs.

中文翻译：

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饲料中添加鱼油替代鱼油对幼体梭子蟹（Portunus trituberculatus）生长性能，抗氧化酶活性和组织脂肪酸组成的影响

进行了为期8周的喂养试验，以评估用混合植物油（VOs）代替鱼油（FO）对游泳蟹Portunustrituberculatus的生长性能，抗氧化酶活性和脂肪酸组成的影响。配制了五种等氮和等脂日粮以包含VOs（菜籽油：棕榈油：亚麻子油= 4：2：1）代替0（对照日粮），250、500、750和1000 g / kg FO（定义为D0 ，D25，D50，D75，D100）。随机放养300只幼蟹（初始重量2.34±0.08 g），并分入三个水泥池中的300个单独的矩形塑料篮中。每种处理方法有三个重复样本，一个重复样本有20个游泳蟹，每个饮食喂食60只螃蟹，分布在60个篮子中。结果表明，饲喂对照日粮的蟹比饲喂D75和D100 VO日粮的蟹显示出更高的存活率，最终体重，体重增加百分比（PWG），比生长速率和换羽频率，肌肉中的粗蛋白和粗脂含量。 （p <.05）。喂养D25 VO日粮的螃蟹的血淋巴中甘油三酸酯，低密度脂蛋白胆固醇和总蛋白的浓度显着高于对照饮食日粮的那些（p  <.05） 。肝胰腺中的脂肪酸组成与饮食组成呈正相关。总之，根据PWG，用VO替代FO的最佳估计为250 g / kg。这些发现表明，与VOs相比，游泳蟹对FO的利用更好。