Virus Research ( IF 5 ) Pub Date : 2020-06-20 , DOI: 10.1016/j.virusres.2020.198064 Tingting Ren 1 , Yanxue Wang 2 , Haotai Chen 1 , Kailing Wang 1 , Xin Gao 1 , Lei Liu 3 , Yongguang Zhang 1 , Yuefeng Sun 1
MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/2009 influenza A virus replication by targeting viral HA and NS. However, whether microRNA ssc-miR-4331-5p (miR-4331-5p) regulates foot and mouth virus (FMDV) replication remains unclear. To explore the role of miR-4331-5p in FMDV infection, we detected the expression level of miR-4331-5p in porcine kidney (PK-15) cells. The results showed that FMDV infection directly upregulates miR-4331-5p expression, while transfection of mimics or inhibitor of miR-4331-5p promotes or inhibits FMDV replication. Further investigation clearly showed that miR-4331-5p increases FMDV replication through inhibiting type I interferon pathways. These data demonstrate that miR-4331-5p plays an important role in regulating FMDV replication.
中文翻译:
MicroRNA-4331-5p通过抑制PK-15细胞中的干扰素途径来促进FMDV复制。
在病毒攻击过程中,MicroRNA在调节病原体与宿主细胞之间的战斗中起着至关重要的作用。MiR-4331加重了可传播的胃肠炎病毒(TGEV)诱导的线粒体损伤,并通过靶向细胞CDCA7抑制了TGEV基因7的转录。否则,miR-4331-5p通过靶向病毒HA和NS影响H1N1 / 2009甲型流感病毒复制。但是,microRNA ssc-miR-4331-5p(miR-4331-5p)是否调节口蹄疫病毒(FMDV)复制尚不清楚。为了探索miR-4331-5p在FMDV感染中的作用,我们检测了miR-4331-5p在猪肾(PK-15)细胞中的表达水平。结果表明,FMDV感染直接上调miR-4331-5p的表达,而miR-4331-5p的模拟物或抑制剂的转染则促进或抑制FMDV复制。进一步的研究清楚地表明,miR-4331-5p通过抑制I型干扰素途径增加FMDV复制。这些数据表明,miR-4331-5p在调节FMDV复制中起重要作用。