Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesized MAC-ZIF-8 has good crystallinity, thermal stability and high surface area which made it suitable for immobilization of nano-BCL. The MAC-ZIF-8 immobilized nano-BCL (nano-BCL@MAC-ZIF-8) demonstrated high enzyme activity (4.3778 U) and can be recovered and reused for consecutive hydrolysis reaction. It is found that the nano-BCL is well encapsulated with the macropores of the MAC-ZIF-8 structures and they are able to preserve their native configuration with high affinity for substrates (lower Km of 1.068 mM as compared to that of nano-BCL@ZIF-8). The nano-BCL@MAC-ZIF-8 can also be used to fabricate electrochemical biosensors for detection of nitrogenous diphenyl ether pesticides (nitrofen) in real food systems (apricots). The fabricated biosensors demonstrated wide linear range (0−114µM), low limit of detection (0.46µM) and good recovery rate. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, low cost, and better linear range.

游离的生物催化剂如脂肪酶无法回收和再利用；在恶劣的反应条件下极不稳定。本研究旨在合成分级有序的宏微孔ZIF-8结构（MAC-ZIF-8），用于封装纳米伯克霍尔德菌原脂肪酶（纳米BCL）。合成的MAC-ZIF-8具有良好的结晶度，热稳定性和高表面积，使其适合于纳米BCL的固定化。固定化MAC-ZIF-8的纳米BCL（nano-BCL @ MAC-ZIF-8）具有很高的酶活性（4.3778 U），可以回收再用于后续的水解反应。发现纳米BCL被MAC-ZIF-8结构的大孔很好地封装，并且它们能够以对底物的高亲和力保持其天然构型（与纳米BCL相比，较低的Km为1.068 mM @ ZIF-8）。nano-BCL @ MAC-ZIF-8还可用于制造电化学生物传感器，以检测实际食品系统（杏子）中的含氮二苯醚农药（nitrofen）。制成的生物传感器显示出宽线性范围（0-114µM），低检测限（0。46µM）和良好的回收率。与传统的检测方法相比，所制造的生物传感器还具有易于操作，成本低和线性范围更好的优点。