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SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability
Plant Science ( IF 5.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.plantsci.2020.110551
Tomáš Vyhnánek 1 , Eva Nevrtalová 1 , Marie Bjelková 2 , Barbora Balgová 1
Affiliation  

Our study aimed to optimize a selection of a suitable combination of SSRs (Simple Sequence Repeats) for determination of technical Cannabis cultivars and genetic variability level. We used sequences of 23 published SSR families (107 alleles) and amplified them in 28 cultivars. One of the alleles possesses no selective information (SSR family CAN1660) due to its presence in every single tested cultivar. We excluded it, together with another 11 least informative alleles. After data filtration, we used 96 alleles to do recursive sub-sampling of random alleles' sets. We found a minimal set of 8 alleles (in three different combinations) to distinguish 28 analyzed cultivars from each other. Our results contribute to saving resources and to reduce the performance time of the molecular analysis.

中文翻译:

工业大麻品种的 SSR 基因座调查:优化成本效益分析以研究遗传变异

我们的研究旨在优化选择合适的 SSR(简单序列重复)组合,以确定技术大麻品种和遗传变异水平。我们使用了 23 个已发表的 SSR 家族(107 个等位基因)的序列,并在 28 个栽培品种中对其进行了扩增。其中一个等位基因不具有选择性信息(SSR 家族 CAN1660),因为它存在于每个测试品种中。我们排除了它,以及另外 11 个信息量最少的等位基因。数据过滤后,我们使用 96 个等位基因对随机等位基因集进行递归子采样。我们发现了一组最小的 8 个等位基因(以三种不同的组合)来区分 28 个分析的栽培品种。我们的结果有助于节省资源并减少分子分析的执行时间。
更新日期:2020-09-01
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