Functional imaging in behaving animals is essential to explore brain functions. Real-time optical imaging of brain functions is limited by light scattering, skull distortion, timing resolution and subcellular precision that altogether, make challenging the rapid acquisition of uncorrupted functional data of cells integrated de novo in the neurogliovascular unit. We report multimodal transcranial in vivo optical imaging for the fast and direct visualization of microcirculation in the perfusion domain where new cells incorporated in the neurogliovascular unit during the progression of a seizure disorder and its treatment. Using this methodology, we explored the performance improvement of cells integrated de novo in the neurogliovascular unit. We report fast transcranial imaging of blood microcirculation at sites of pericyte turnover in the epileptic brain and after treatment with a trophic factor that revealed key features of the regenerating neurogliovascular unit.

行为动物的功能成像对于探索大脑功能至关重要。脑功能的实时光学成像受到光散射，颅骨畸变，时间分辨率和亚细胞精度的限制，这些都使快速获取神经胶质血管单元中新生细胞的完整功能数据具有挑战性。我们报告多模式经颅体内光学成像的快速和直接可视化的灌注领域，在癫痫病及其治疗过程中纳入神经胶质血管单位的新细胞的灌注域。使用这种方法，我们探索了从头开始集成电池的性能改进在神经胶质血管单位。我们报告了癫痫大脑中周细胞周转部位血液微循环的快速经颅成像，并用营养因子治疗后揭示了再生神经胶质血管单位的关键特征。