Semi-artificial photosynthesis (biohybrid) provides an intriguing opportunity for efficient CO₂-to-CH₄ conversion. However, creating a desirable semiconductor in biohybrids remains a great challenge. Here, by doping Ni into CdS nanoparticles, we have successfully developed the Methanosarcina barkeri-Ni:CdS biohybrids. The CH₄ yield by the M. barkeri-Ni_(0.75%):CdS biohybrids was approximately 250% higher than that by the M. barkeri-CdS biohybrids. The suitable Ni dopants serve as an effective electron sink, which accelerates the photoelectron transfer in biohybrids. In addition, Ni doping changes the metabolic status of M. barkeri and results in a higher expression of a series of proteins for electron transfer, energy conversion, and CO₂ fixation. These increased proteins can promote the photoelectron capture by M. barkeri and injection into cells, which trigger a higher intracellular reduction potential to drive the reduction of CO₂ to CH₄. Our discovery will offer a promising strategy for the optimization of biohybrids in the solar-to-chemical conversion.

半人工光合作用（生物杂化）为有效地将CO ₂转化为CH ₄提供了有趣的机会。然而，在生物杂交物中创建理想的半导体仍然是巨大的挑战。在这里，通过将Ni掺杂到CdS纳米粒子中，我们已经成功开发了甲烷甲烷八叠球菌-Ni：CdS生物杂化物。的CH ₄收率由M.巴克-Ni _{（0.75％）} ：硫化镉biohybrids比由高约250％M.巴克-CdS biohybrids。合适的镍掺杂剂可作为有效的电子吸收体，从而加速生物杂化物中的光电子转移。此外，Ni掺杂会改变巴氏支原体的代谢状态并导致一系列用于电子转移，能量转换和CO ₂固定的蛋白质的更高表达。这些增加的蛋白质可以促进巴氏支原体捕获光电子并注入细胞，从而触发更高的细胞内还原能力，从而将CO ₂还原为CH ₄。我们的发现将为优化太阳能到化学转化中的生物杂交提供有希望的策略。