Antibiotic-loaded nano-delivery systems offer an advanced approach to overcome several limitations associated with antibiotic therapy. Antibiotic-loaded nanofibers can be applied topically for skin and wound healing, post operation implants for the prevention of abdominal adhesion, and prophylaxis and treatment of infections in orthopedic surgery. Here, the authors report the development of local antibiotic delivery system using chitosan- polyethylene oxide (PEO) nanofibers for delivery of teicoplanin. Successful electrospinning of chitosan-PEO solution containing 2 and 4 w/v% teicoplanin resulted in uniform and bead-less nanofibers. Nanofibers were able to release teicoplanin up to 12 days. Antibacterial test in agar diffusion and time-kill study on Staphylococcus aureus also demonstrate that loading teicoplanin in chitosan-PEO nanofibers not only kept the antibacterial activity of antibiotic but also, enhanced it up to 1.5 to 2 fold. Teicoplanin loaded nanofibers did not show any cytotoxicity to human fibroblast. Moreover, in vivo study on rat full thickness wound model confirmed safety and efficacy of applying teicoplanin loaded nanofibers and significant improve in wound closure was observed especially with nanofibers containing 4% teicoplanin. The sustained release profile, enhanced drug activity, cytocompatibility, and significant wound healing activity affirm the potential applications of teicoplanin-loaded nanofibers in wound healing and local antibiotic delivery.

载有抗生素的纳米递送系统提供了一种先进的方法来克服与抗生素治疗相关的一些限制。载有抗生素的纳米纤维可局部用于皮肤和伤口愈合，术后植入物可预防腹部粘连，并在整形外科手术中预防和治疗感染。在这里，作者报告了使用壳聚糖-聚环氧乙烷（PEO）纳米纤维递送替考拉宁的局部抗生素递送系统的开发。包含2和4 w / v％替考拉宁的壳聚糖-PEO溶液的成功静电纺丝产生均匀且无珠的纳米纤维。纳米纤维能够释放替考拉宁长达12天。金黄色葡萄球菌在琼脂扩散中的抗菌试验和杀灭时间的研究还证明在壳聚糖-PEO纳米纤维中加入替考拉宁不仅可以保持抗生素的抗菌活性，而且可以将抗生素的抗菌能力提高1.5到2倍。载有替考拉宁的纳米纤维对人成纤维细胞没有任何细胞毒性。此外，对大鼠全层伤口模型的体内研究证实了应用替考拉宁负载的纳米纤维的安全性和有效性，并且观察到伤口闭合的显着改善，尤其是对于含有4％替考拉宁的纳米纤维。持续释放特性，增强的药物活性，细胞相容性和显着的伤口愈合活性证实了载有替考拉宁的纳米纤维在伤口愈合和局部抗生素递送中的潜在应用。