In this study, we report an industrially feasible conversion of wood hydrolysate from a pilot-scale forest biorefinery. The wood hydrolysate was compared to a synthetic hydrolysate with a similar content of carbon sources (glucose, xylose, and acetate). To maximize the PHB concentration, high-cell density cultivations were conducted in bioreactors for 52 h using the bacterium Paraburkholderia sacchari IPT 101 LMG 19450. The conversion of wood hydrolysate yielded a maximum PHB concentration of 34.5 g/L, which is among the highest reported for lignocellulose hydrolysates. In comparison, the use of a synthetic hydrolysate resulted in substantially lower PHB concentration (22.0 g/L). This could be attributed to a higher maximum specific growth rate of 0.36 vs. 0.33 per h for cells grown in wood hydrolysate vs. synthetic hydrolysate. For the wood hydrolysate, the final PHB content per cell mass reached 58 % g/g and a maximum PHB productivity of 0.72 g/(Lh), while the synthetic hydrolysate reached 55 % g/g and 0.46 g/(Lh) respectively. Given the increase in bacterial growth and PHB productivity when using the wood hydrolysate, the chosen lignocellulose conversion process is beneficial for subsequent biotechnological conversion to key bioproducts.

在这项研究中，我们报告了从中试规模的森林生物精炼厂转换木材水解产物的工业可行性。将木材水解产物与具有相似碳源（葡萄糖，木糖和乙酸盐）含量的合成水解产物进行比较。为了使PHB浓度最大化，使用细菌糖衣芽孢杆菌在生物反应器中进行了52小时的高细胞密度培养IPT 101 LMG19450。木材水解产物的转化产生的最大PHB浓度为34.5 g / L，这是报道的木质纤维素水解产物的最高浓度。相比之下，使用合成水解物可大大降低PHB浓度（22.0 g / L）。这可以归因于在木材水解产物与合成水解产物中生长的细胞更高的最大比生长速率，为0.36 vs. 0.33每小时。对于木材水解产物，每细胞质量的最终PHB含量达到58％g / g，最大PHB生产率为0.72 g /（Lh），而合成水解产物分别达到55％g / g和0.46 g /（Lh）。考虑到使用木材水解物时细菌生长和PHB生产率的提高，选择的木质纤维素转化工艺有利于后续生物技术转化为关键生物产品。