The presence of anti-Ascaris (anti-Asc) immunoglobin isotypes alters the risk of allergic asthma. In this study, we analyzed the relationships between serum levels of anti-Asc IgE, IgG1, and IgG4, without concurrent infection by the parasite, and the presence of asthma. We measured cytokine levels from Th1, Th2, and Th17 profiles. Children aged 2–14 years old, asthmatics (n = 64), and non-asthmatics (n = 40) were selected according to the International Study of Asthma and Allergies in Childhood criteria. Asthmatic patients who had positive skin allergy tests were considered to have allergic asthma. Stool exams were performed to exclude children who were parasitized by helminths/protozoans and blood samples were collected in non-parasitized individuals. We performed peripheral blood leukocyte counts and in vitro culture following mitogenic stimulation. Levels of cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17) in the supernatants were measured using a cytometric bead array. Titration of serum total IgE and IgE specific to Ascaris were obtained using ImmunoCAP; IgG1 and IgG4 titers were measured using enzyme-linked immunosorbent assays. Anti-Asc IgE was associated with a higher risk of asthma and an increase in the number of eosinophils and neutrophils. By contrast, anti-Asc IgG1 could be considered a protective factor against asthma, associated with lower levels of circulating neutrophils. There were high levels of IL-6 and TNF-α in asthmatics. Levels of IL-6, but not TNF-α, depended on the presence of anti-Asc IgG1 in serum. Anti-Asc IgE appears to increase risk of asthma, and anti-Asc IgG1 appears to favor decreased neutrophil counts and increased IL-6 levels.

抗蛔虫（抗 Asc）免疫球蛋白同种型的存在改变了过敏性哮喘的风险。在这项研究中，我们分析了抗 Asc IgE、IgG1 和 IgG4 的血清水平（没有寄生虫并发感染）与哮喘的存在之间的关系。我们测量了 Th1、Th2 和 Th17 谱中的细胞因子水平。根据儿童哮喘和过敏症国际研究标准选择 2-14 岁儿童、哮喘患者 (n = 64) 和非哮喘患者 (n = 40)。皮肤过敏试验阳性的哮喘患者被认为患有过敏性哮喘。进行粪便检查以排除被蠕虫/原生动物寄生的儿童，并在非寄生个体中收集血液样本。我们进行了外周血白细胞计数和促有丝分裂刺激后的体外培养。使用细胞计数珠阵列测量上清液中细胞因子（IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ和IL-17）的水平。血清总IgE和蛔虫特异性IgE滴定使用 ImmunoCAP 获得；使用酶联免疫吸附测定法测量 IgG1 和 IgG4 滴度。抗 Asc IgE 与较高的哮喘风险以及嗜酸性粒细胞和中性粒细胞数量的增加有关。相比之下，抗 Asc IgG1 可被视为哮喘的保护因子，与循环中性粒细胞水平较低有关。哮喘患者的 IL-6 和 TNF-α 水平较高。IL-6 的水平，而不是 TNF-α，取决于血清中抗 Asc IgG1 的存在。抗 Asc IgE 似乎会增加哮喘风险，而抗 Asc IgG1 似乎有利于减少中性粒细胞计数和增加 IL-6 水平。