The objective of this study is to formulate stable double emulsions of the water in oil in water (W₁/O/W₂) type containing a bioactive peptide (BP). The effects of the following parameters were investigated: the mechanical emulsification devices (high-speed homogenizer (HSH) ultrasonicator (US), microfluidizer (MF), and combinations of HSH with US or MF), the BP concentration (0–20 %), and the hydrophilic emulsifiers (Tween 80, chitosan, and a mixture of Tween 80 and chitosan) for the external aqueous phase on the droplet storage stability of double emulsions. The double emulsions with the optimum formation showed small droplet size, high encapsulation efficiency and good storage stability when 5% BP loading and the combination of high-speed homogenizer and microfluidizer was applied. Moreover, double emulsions stabilized by emulsifier-Tween 80 layers displayed excellent physical stability without phase separation. The double emulsions stabilized by an emulsifier-chitosan layer contained positively charged droplets and a thick layer in the external aqueous phase. However, double emulsion stabilized by the mixture of Tween 80 and chitosan emulsifiers showed a bi-modal size distribution, indicating that a synergistic interaction did not exist between Tween 80 and chitosan. The information obtained from this study is useful for preparing stable functional food or cosmetic products from double emulsions using a BP.

这项研究的目的是配制水包油水中稳定的水双重乳液（W ₁ / O / W ₂）类型包含生物活性肽（BP）。研究了以下参数的影响：机械乳化装置（高速均化器（HSH）超声仪（US），微流化器（MF）以及HSH与US或MF的组合），BP浓度（0-20％） ，以及亲水性乳化剂（吐温80，壳聚糖以及吐温80和壳聚糖的混合物）对于外部水相对双乳状液的液滴储存稳定性的影响。当使用5％的BP负载并结合使用高速均质器和微流化器时，具有最佳形成的双乳化液显示出较小的液滴尺寸，较高的包封效率和良好的储存稳定性。此外，通过乳化剂-吐温80层稳定的双重乳液显示出优异的物理稳定性，而不会发生相分离。由乳化剂-壳聚糖层稳定的双重乳液在外部水相中包含带正电荷的液滴和厚层。然而，由吐温80和壳聚糖乳化剂的混合物稳定的双乳液显示出双峰尺寸分布，表明吐温80和壳聚糖之间不存在协同作用。从这项研究中获得的信息可用于使用BP从双乳液制备稳定的功能性食品或化妆品。