To investigate the effects of quercetin on cell viability and apoptosis in meningioma cells and to determine the underlying molecular mechanism. HBL-52 meningioma cells were treated with quercetin at doses of 1, 5, 10, 20, and 40 ng/mL for 24, 36 and 48 h, and cell viability was assessed using the Cell Counting kit-8 (CCK-8) test. Apoptosis was determined by flow cytometry. Bax, Bcl-2, and IGFBP5 protein expression was assessed by western blot, and IGFBP5 and miR-197 mRNA levels were measured using quantitative reverse transcription PCR (qRT-PCR). The interaction between miR-197 and IGFBP5 was verified by dual luciferase assay. Quercetin reduces viability and proliferation and increases apoptosis in HBL-52 cells in a dose- and time-dependent manner. Quercetin treatment also decreases Bcl-2 and increases Bax protein expression, and increases miR-197 mRNA while reducing IGFBP5 mRNA expression. A dual luciferase assay showed that miR-197 interacts directly with binding sites in the 3′untranslated region of IGFBP5, and that miR-197 overexpression reduced IGFBP5 expression. Quercetin may reduce meningioma cell proliferation and increase apoptosis by activating the miR-197/IGFBP5 cascade and regulating Bcl-2/Bax.

研究槲皮素对脑膜瘤细胞活力和凋亡的影响，并确定其潜在的分子机制。用槲皮素分别以1、5、10、20和40 ng / mL的剂量处理HBL-52脑膜瘤细胞24、36和48小时，并使用Cell Counting kit-8（CCK-8）评估细胞活力测试。通过流式细胞术确定细胞凋亡。通过蛋白质印迹评估Bax，Bcl-2和IGFBP5蛋白表达，并使用定量逆转录PCR（qRT-PCR）测量IGFBP5和miR-197 mRNA水平。通过双重荧光素酶测定法验证了miR-197和IGFBP5之间的相互作用。槲皮素以剂量和时间依赖性方式降低HBL-52细胞的活力和增殖并增加其凋亡。槲皮素治疗还可以降低Bcl-2并增加Bax蛋白表达，并增加miR-197 mRNA的表达，同时降低IGFBP5 mRNA的表达。双重萤光素酶检测显示miR-197与蛋白3'非翻译区的结合位点直接相互作用IGFBP5和那miR-197过表达减少了IGFBP5表达。槲皮素可通过激活miR-197 / IGFBP5级联并调节Bcl-2 / Bax来减少脑膜瘤细胞增殖并增加凋亡。