Treated fish wastes have found many applications in industry and medicine. Besides, nowadays low-cost scaffold with antimicrobial activity which can accelerates the process of wound healing is very demanding. In this study fish swim bladder (FSB), taken from Rutilus frisii, which is a disposable waste was doped with silver nanoparticles (AgNPs) and evaluated as antimicrobial wound dressing. The scanning electron microscopy (SEM) micrographs showed the presence of AgNPs on the scaffold. Histological observation confirmed cells and muscle removal from FSB and collagen preservation. There was significant antibacterial activity even in 50 ppm AgNPs concentration against pathogenic bacteria, swelling ratio was rather low, and cytotoxic assay revealed that the AgNPs-FSB scaffold had no toxic effect on human foreskin fibroblast (HFF) cells. Interestingly, despite the porous structure, the AgNPs-FSB scaffold was found to be a suitable barrier to microbial penetration even after 72 h. Further study showed the gradual release of AgNPs during 24 h. In conclusion, biofabricated FSB prepared in this study have appropriate characteristics notably encompassing a high quantity of collagen and broad-spectrum antimicrobial activity. Also, its porous structure made it suitable as a 3-D structure for the growth of cells and adding other antimicrobial nano-sized materials.

经处理的鱼废料在工业和医学中发现了许多应用。此外，现今对具有抗菌活性，可以加速伤口愈合过程的低成本支架的要求很高。在这项研究中，鱼R取自Rutilus frisii一次性废物中掺有银纳米颗粒（AgNPs），并被评估为抗菌伤口敷料。扫描电子显微镜（SEM）显微照片显示支架上存在AgNP。组织学观察证实从FSB和胶原保存中去除了细胞和肌肉。即使在浓度为50 ppm的AgNPs对病原菌的情况下，仍具有显着的抗菌活性，溶胀率相当低，并且细胞毒性试验表明，AgNPs-FSB支架对人包皮成纤维细胞（HFF）没有毒性作用。有趣的是，尽管具有多孔结构，但即使在72小时后，AgNPs-FSB支架仍是微生物渗透的合适屏障。进一步的研究表明，AgNPs在24小时内逐渐释放。结论，在这项研究中制备的生物制造的FSB具有适当的特性，尤其包括大量的胶原蛋白和广谱抗菌活性。而且，其多孔结构使其适合作为3D结构用于细胞生长和添加其他抗菌纳米级材料。