Abstract Background Liquid wax esters are widely used in cosmetic as well as pharmaceutical and other industries. The demand of organic and natural products is increasing nowadays. Coconut oil has benefit fatty acids and mainly used for oil-based and moisturizer products. Liquid wax esters from coconut oil and unsaturated fatty alcohol can be synthesized by enzymatic reaction; and interesting for using as an alternative natural ingredient in these industries. Results Optimal condition for coconut oil based wax ester synthesis by immobilized lipase EQ3 was 10 U of enzyme, temperature at 30°C and molar ratio of coconut oil to oleyl alcohol at 1:3 (mol/mol) (0.33X) dissolved in isooctane for 12 h, while for Lipozyme RM IM optimal condition was 10 U of enzyme, temperature at 45°C and oil/alcohol molar ratio at 1:3 (0.33X) dissolved in isooctane for 3 h. Percentage of wax esters synthesized by both lipases reached more than 88%. Both immobilized lipases catalyzed high yield of wax esters within the 2nd batch; after that, the immobilized lipases showed reduced activity and synthesized Conclusions The liquid wax ester synthesis by commercial Lipozyme RM IM had higher effect than immobilized lipase EQ3, but both catalysts were stable within 2 batches in the optimum condition. The characteristic properties of wax esters showed potential for use as components in cosmetics and skin care products.

中文翻译：

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用固定化脂肪酶 EQ3 和商业脂酶 RMIM 酶促合成椰子油基蜡酯

摘要 背景液体蜡酯广泛用于化妆品以及制药等行业。如今，对有机和天然产品的需求正在增加。椰子油具有有益脂肪酸，主要用于油基和保湿产品。椰子油与不饱和脂肪醇可通过酶促反应合成液态蜡酯；在这些行业中用作替代天然成分很有趣。结果 通过固定化脂肪酶 EQ3 合成椰子油基蜡酯的最佳条件是 10 U 的酶、30°C 的温度和溶解在异辛烷中的椰子油与油醇的摩尔比为 1:3 (mol/mol) (0.33X) 12 小时，而 Lipozyme RM IM 的最佳条件是 10 U 的酶、45°C 的温度和 1:3 的油/醇摩尔比 (0. 33X) 溶解在异辛烷中 3 小时。两种脂肪酶合成蜡酯的比例均达到88%以上。在第二批中，两种固定化脂肪酶都催化了蜡酯的高产率；之后，固定化脂肪酶活性降低，合成结果 结论 市售 Lipozyme RM IM 合成液体蜡酯的效果高于固定化脂肪酶 EQ3，但两种催化剂在最佳条件下在 2 个批次内均保持稳定。蜡酯的特性显示出用作化妆品和护肤产品成分的潜力。固定化脂肪酶活性降低，合成结果 结论 市售 Lipozyme RM IM 合成液体蜡酯的效果高于固定化脂肪酶 EQ3，但两种催化剂在最佳条件下在 2 个批次内均保持稳定。蜡酯的特性显示出用作化妆品和护肤产品成分的潜力。固定化脂肪酶活性降低，合成结果 结论 市售 Lipozyme RM IM 合成液体蜡酯的效果高于固定化脂肪酶 EQ3，但两种催化剂在最佳条件下在 2 个批次内均保持稳定。蜡酯的特性显示出用作化妆品和护肤产品成分的潜力。