The effects of quinoid compounds on azo dyes decolorization were studied. Compared with other quinones, menadione was the most effective at aiding azo dye decolorization. Sodium formate was a suitable carbon source for the anaerobic decolorization system. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis indicated that the microbial structure changed in response to varying carbon sources. Phylogenetic analysis showed that the anaerobic sludge was consisted mainly of nine genera. The mechanism studies showed that the biotransformation of menadione to its hydroquinone form was the rate-limiting step in the dye decolorization process. Moreover, study of the electron transfer mechanism of quinone-mediated reduction showed that azo dye decolorization is not a specific reaction. The NADH chain was involved in the decolorization process. The methane production test indicated that azo dyes had an inhibitory effect on methane production. However, supplementation with a redox mediator could recover the inhibited methanogenesis. High-throughput sequencing analysis revealed that the methanogenic archaeal community was altered in the anaerobic sludge with or without azo dyes and the redox mediator.

研究了醌类化合物对偶氮染料脱色的影响。与其他醌相比，甲萘醌在帮助偶氮染料脱色方面最有效。甲酸钠是厌氧脱色系统的合适碳源。聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析表明，微生物结构随着碳源的变化而变化。系统发育分析表明，厌氧污泥主要由9个属组成。机理研究表明，甲萘醌向氢醌形式的生物转化是染料脱色过程中的限速步骤。此外，对醌介导的还原反应的电子转移机理的研究表明，偶氮染料的脱色不是特定的反应。NADH链参与了脱色过程。甲烷产生试验表明，偶氮染料对甲烷产生具有抑制作用。但是，补充氧化还原介体可以恢复抑制的甲烷生成。高通量测序分析表明，在有或没有偶氮染料和氧化还原介体的厌氧污泥中，产甲烷古菌群落发生了变化。