The transcription factor Nrf2 and its negative regulator Keap1 play important roles in the maintenance of redox homeostasis in animal cells. Nrf2 activates defenses against oxidative stress and xenobiotics. Homologs of Nrf2 and Keap1 are present in Drosophila melanogaster (CncC and dKeap1, respectively). The aim of this study was to explore effects of CncC deficiency (due to mutation in the cnc gene) or enhanced activity (due to mutation in the dKeap1 gene) on redox status and energy metabolism of young adult flies in relation to behavioral traits and resistance to a number of stressors. Deficiency in either CncC or dKeap1 delayed pupation and increased climbing activity and heat stress resistance in 2-day-old adult flies. Males and females of the ∆keap1 line shared some similarities such as elevated antioxidant defense as well as lower triacylglyceride and higher glucose levels. Males of the ∆keap1 line also had a higher activity of hexokinase, whereas ∆keap1 females showed higher glycogen levels and lower values of respiratory control and ATP production than flies of the control line. Mutation of cnc gene in allele cnc^EY08884 caused by insertion of P{EPgy2} transposon in cnc promotor did not affect significantly the levels of metabolites and redox parameters, and even activated some components of antioxidant defense. These data suggest that the mutation can be hypomorphic as well as CncC protein can be dispensable for adult fruit flies under physiological conditions. In females, CncC mutation led to lower mitochondrial respiration, higher hexokinase activity and higher fecundity as compared with the control line. Either CncC activation or its deficiency affected stress resistance of flies.

转录因子Nrf2及其负调控因子Keap1在维持动物细胞氧化还原稳态中起重要作用。Nrf2激活抵抗氧化应激和异种生物的防御能力。Nrf2和Keap1的同源物存在于果蝇（Dncophila melanogaster）中（分别为CncC和dKeap1）。这项研究的目的是探讨CncC缺乏（由于cnc基因的突变）或增强的活性（由于dKeap1基因的突变）对年轻成年果的氧化还原状态和能量代谢与行为特征和抵抗力的关系。对许多压力源。CncC或dKeap1的缺乏会延缓化up，并在2天大的成年果蝇中增加攀爬活动和抗热应激能力。男女之∆keap1品系具有一些相似之处，例如抗氧化剂防御能力提高，甘油三酯含量较低和葡萄糖含量较高。keap1品系的雄性己糖激酶活性也较高，而keap1品系的雌性糖原水平更高，呼吸控制和ATP产生的值也低于对照组。的突变数控在等位基因基因CNC ^EY08884引起的插入p {EPgy2}转座子在数控启动子并没有显着影响代谢物的水平和氧化还原参数，甚至激活了抗氧化防御的某些成分。这些数据表明，该突变可能是亚型的，而CncC蛋白对于在生理条件下的成年果蝇是必不可少的。在女性中，与对照组相比，CncC突变导致线粒体呼吸降低，己糖激酶活性更高和繁殖力更高。CncC激活或其缺陷都会影响果蝇的抗逆性。