With depletion of fossil fuel, microalgae is considered as a promising substitute due to high growth rate, efficient cost and high biofuels content. This study investigated the effect of temperature on mixotrophic cultivation of Chlorella vulgaris. In addition, the combination carbon source of inorganic (HCO₃⁻ or CO₃²⁻) and organic (glucose or acetate) for microalgae cultivation was evaluated to obtain the optimum carbon source for mixotrophic cultivation. The results showed that the optimum temperature of microalgae cultivation was at the range of 15–20 °C. The activity of Rubisco was obviously inhibited at the temperature of 30 °C, however, citrate synthase was not susceptible to the increasing temperature. COD removal efficiency was all higher than 64.0%. Low temperature was benefit for protein formation, and the lipid accumulation occurred at high temperature. The results provide a fresh perspective between enzyme activity and temperature variation for product accumulation of microalgae.

随着化石燃料的枯竭，微藻由于其高增长率，有效成本和高生物燃料含量而被认为是有前途的替代品。本研究考察了温度对普通小球藻混养的影响。此外，无机组合碳源（HCO ₃ ^-或CO ₃ ^2-），并评估了用于微藻培养的有机（葡萄糖或乙酸盐）以获得用于混合营养培养的最佳碳源。结果表明，微藻培养的最佳温度为15–20°C。Rubisco的活性在30°C的温度下明显受到抑制，但是，柠檬酸合酶对温度升高不敏感。COD去除率均高于64.0％。低温有利于蛋白质的形成，脂质积累在高温下发生。结果为微藻产物的积累提供了酶活性和温度变化之间的新视角。