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Biosynthesis of natural colorant by Talaromyces amestolkiae: mycelium accumulation and colorant formation in incubator shaker and in bioreactor
Biochemical Engineering Journal ( IF 3.9 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.bej.2020.107694
Fernanda de Oliveira , Laura Carmona Ferreira , Álvaro Baptista Neto , Maria Francisca Simas Teixeira , Valéria de Carvalho Santos Ebinuma

Abstract Fungi bioproducts have recently become commercially important to the food and pharmaceutical industry. However, different structural forms exhibited by filamentous fungi throughout their life cycles are often a bottleneck for productivity in many industrial processes. In this work, the relationship among mycelium accumulation, morphology, and colorant production was evaluated. Experiments were performed in an incubator shaker and in a stirred-tank bioreactor. First, a series of nitrogen sources and pure vitamins were investigated as additives to the monosodium glutamate-glucose medium in order to improve Talaromyces amestolkiae red colorant production in the incubator shaker. MSG and pH played an important role in generating stress, which affected cell growth and colorants production. The colorant synthesis seems to be is triggered under metabolic stress. Next, the effects of starter culture composition (MSG-glucose and nitrogen complex starter media) and airflow (2.0 and 8.0 Lmin−1) were investigated in the bioreactor. After 120 h of cultivation in the bioreactor, a maximum red colorant production of 9.33 UA500nm, 72.38 % glucose consumption, and 4.53 g L-1 biomass concentration were achieved under complex nitrogen as starter medium at 8 Lmin−1. Growth remained at moderate level, probably affected by the synergistic effect of MSG and pH as stress factors. Moreover, pellets formation favored suitable oxygenation of the culture broth leading to the best consumption of the carbon source.

中文翻译:

Talaromyces amestolkiae 生物合成天然着色剂:培养摇床和生物反应器中菌丝体的积累和着色剂的形成

摘要 真菌生物制品最近对食品和制药行业具有重要的商业意义。然而,丝状真菌在其整个生命周期中表现出的不同结构形式通常是许多工业过程中生产力的瓶颈。在这项工作中,评估了菌丝体积累、形态和着色剂产生之间的关系。实验在培养摇床和搅拌罐生物反应器中进行。首先,研究了一系列氮源和纯维生素作为谷氨酸钠-葡萄糖培养基的添加剂,以提高培养摇床中 Talaromyces amestolkiae 红色着色剂的产量。味精和 pH 在产生压力方面发挥了重要作用,这会影响细胞生长和着色剂的产生。着色剂合成似乎是在代谢压力下触发的。接下来,在生物反应器中研究了发酵剂培养物组成(味精-葡萄糖和氮复合发酵剂培养基)和气流(2.0 和 8.0 Lmin-1)的影响。在生物反应器中培养 120 小时后,在 8 Lmin-1 的复合氮作为起始培养基下,获得了 9.33 UA500nm 的最大红色着色剂产量、72.38% 的葡萄糖消耗和 4.53 g L-1 生物量浓度。生长保持在中等水平,可能受到味精和 pH 作为胁迫因素的协同作用的影响。此外,球团形成有利于培养液的适当氧化,导致碳源的最佳消耗。0 Lmin-1) 在生物反应器中进行了研究。在生物反应器中培养 120 小时后,在 8 Lmin-1 的复合氮作为起始培养基下,获得了 9.33 UA500nm 的最大红色着色剂产量、72.38% 的葡萄糖消耗和 4.53 g L-1 生物量浓度。生长保持在中等水平,可能受到味精和 pH 作为胁迫因素的协同作用的影响。此外,球团形成有利于培养液的适当氧化,导致碳源的最佳消耗。0 Lmin-1) 在生物反应器中进行了研究。在生物反应器中培养 120 小时后,在 8 Lmin-1 的复合氮作为起始培养基下,获得了 9.33 UA500nm 的最大红色着色剂产量、72.38% 的葡萄糖消耗和 4.53 g L-1 生物量浓度。生长保持在中等水平,可能受到味精和 pH 作为胁迫因素的协同作用的影响。此外,球团形成有利于培养液的适当氧化,导致碳源的最佳消耗。可能受味精和 pH 作为胁迫因素的协同作用影响。此外,球团形成有利于培养液的适当氧化,导致碳源的最佳消耗。可能受味精和 pH 作为胁迫因素的协同作用影响。此外,球团形成有利于培养液的适当氧化,导致碳源的最佳消耗。
更新日期:2020-09-01
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