The natural production of the native flat oyster Ostrea edulis and the production of seed in hatcheries are insufficient to meet demand. Several projects have recently been developed to optimise flat oyster production and to establish a reliable protocol for broodstock conditioning in hatcheries. In this sense, the positive effect of a gradient of temperature and a gradient of daylight on the gonadal development, spawning and larval production of O. edulis during winter and autumn conditioning was previously confirmed. No other study has yet been optimised the effect of other factors on flat oyster conditioning.

This work examines the combined effect of two important factors on broodstock conditioning, namely the food ration and the water flow rate, for the first time. Furthermore, the bacteriological load of oyster broodstock and its influence on O. edulis conditioning had not been studied before. The results showed that the broodstock conditioned with a food ration of 3% produced the highest number of viable larvae. Oysters conditioned at a flow rate of 3 L h⁻¹ oyster⁻¹ had the highest mortality rate, coinciding with the highest levels of vibrio and heterotrophic bacteria in both meat and intervalvar fluid. The best larval production results were obtained when the 3% food ration was combined with a water flow rate of 2 L h⁻¹ oyster⁻¹.

本地扁平牡蛎可食牡蛎的自然生产和孵化场中种子的生产不足以满足需求。最近开发了几个项目，以优化牡蛎的生产并为孵化场的亲鱼养殖建立可靠的协议。从这个意义上讲，先前已经确认了温度梯度和日光梯度对冬至秋季条件下可食O.edulis的性腺发育，产卵和幼虫产生的积极影响。尚无其他研究优化其他因素对扁平牡蛎调节的影响。

这项工作首次检验了两个重要因素对亲鱼调节的综合影响，即食物定量和水流量。此外，以前没有研究过牡蛎亲鱼的细菌载量及其对可食O.edulis的影响。结果表明，以3％的食物比例调节亲鱼产生的幼虫数量最多。以3 L h ^-1牡蛎^-1的流量调节的牡蛎的死亡率最高，这与肉类和间隔液中的弧菌和异养细菌含量最高有关。当配给3％的食物和2 L h ^-1的牡蛎水流时，可获得最佳的幼体生产结果^-1。