Abstract Aminofunctionalized LAPONITE® RD was employed for efficient chlorhexidine digluconate (CHX-DG) incorporation. The structure and properties of the unloaded (Lap-APTES) and loaded (Lap-APTES:CHX-DG) hybrids were investigated by powder X-ray diffraction (PXRD), thermal analysis, small angle X-ray scattering (SAXS), and infrared absorption spectroscopy (FTIR). A typical adsorption experiment was conducted to evaluate how the parameters contact time and concentration affected CHX-DG incorporation into the clay and showed that this compound had high affinity for Lap-APTES, adsorption equilibrium was reached at 90 min, with CHX-DG incorporation of 1402.7 mg/g. FTIR analysis confirm the interaction by hydrogen bonds between the CHX-DG and APTES amine groups and the laponite OH groups, resulting in materials with potential antibacterial properties for controlled drug release. Even at low concentrations (0.042 mg/g), Lap-APTES:CHX-DG was effective against S. pyogenes. The Lap, Lap-APTES, and Lap-APTES:CHX-DG cytotoxicity to GM07492A cells (human fibroblasts) was investigated by the XTT colorimetric assay, which revealed that CHX-DG incorporation into Lap-APTES reduced drug cytotoxicity. Drug release experiments demonstrated that a maximum of 10% (m/m) CHX-DG was released within 24 h and confirmed the higher affinity between Lap-APTES and CHX-DG.

中文翻译：

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氨基官能化的 LAPONITE® 作为一种用于掺入二葡萄糖酸氯己定的多功能杂化材料：细胞毒性和抗菌活性

摘要 氨基官能化的 LAPONITE® RD 用于有效掺入二葡萄糖酸氯己定 (CHX-DG)。通过粉末 X 射线衍射 (PXRD)、热分析、小角 X 射线散射 (SAXS) 和负载 (Lap-APTES:CHX-DG) 杂化物的结构和性能研究红外吸收光谱 (FTIR)。进行了典型的吸附实验以评估参数接触时间和浓度如何影响 CHX-DG 掺入粘土中，并表明该化合物对 Lap-APTES 具有高亲和力，吸附平衡在 90 分钟时达到，CHX-DG 掺入1402.7 毫克/克。FTIR 分析证实了 CHX-DG 和 APTES 胺基团与锂皂石 OH 基团之间的氢键相互作用，导致材料具有潜在的抗菌特性，可控制药物释放。即使在低浓度 (0.042 mg/g) 下，Lap-APTES:CHX-DG 也能有效对抗化脓性链球菌。通过 XTT 比色法研究了 Lap、Lap-APTES 和 Lap-APTES：CHX-DG 对 GM07492A 细胞（人成纤维细胞）的细胞毒性，结果表明 CHX-DG 掺入 Lap-APTES 降低了药物细胞毒性。药物释放实验表明，在 24 小时内最多释放 10% (m/m) CHX-DG，并证实 Lap-APTES 和 CHX-DG 之间具有更高的亲和力。这表明 CHX-DG 掺入 Lap-APTES 降低了药物细胞毒性。药物释放实验表明，在 24 小时内最多释放 10% (m/m) CHX-DG，并证实 Lap-APTES 和 CHX-DG 之间具有更高的亲和力。这表明 CHX-DG 掺入 Lap-APTES 降低了药物细胞毒性。药物释放实验表明，在 24 小时内最多释放 10% (m/m) CHX-DG，并证实 Lap-APTES 和 CHX-DG 之间具有更高的亲和力。