Starch-accumulating microalgae are a potential source of glucose, a valuable industrial feedstock. However, the recalcitrance of their cell wall hinders the enzymatic hydrolysis of the intracellular starch. This work studied starch accumulation, cell wall disruption, and enzymatic hydrolysis of the green microalga Chlorella sorokiniana. Starch accumulation reached 26% of the microalgal biomass in response to nitrogen starvation. The use of cellulases for cell wall disruption was not successful; however, disruption of the dry biomass using a vibratory mill was very effective for the hydrolysis of the intracellular starch by amylases. In reaction mixtures with a solids loading of 5% (m/m), the starch of milled cells was hydrolyzed by fungal amylases, without a prior gelatinization step, to a final respective glucose yield and concentration of 99% and 16 g.L⁻¹ within 5 h. The increase of the solids loading to 25% (m/m) resulted in a glucose yield and concentration of 75% and 70 g.L⁻¹, respectively, within the same reaction time.

淀粉积累微藻是葡萄糖的潜在来源，葡萄糖是一种有价值的工业原料。但是，它们细胞壁的顽固阻碍了细胞内淀粉的酶水解。这项工作研究了绿色微藻小球藻小球藻的淀粉积累，细胞壁破坏和酶促水解。。响应氮饥饿，淀粉积累达到微藻生物量的26％。使用纤维素酶破坏细胞壁并不成功。然而，使用振动磨破坏干生物质对于淀粉酶水解细胞内淀粉非常有效。在具有5％的固体负载的反应混合物（M / M），精细胞的淀粉是由真菌淀粉酶水解，而无需事先糊化工序中，至最终各自葡萄糖产量和99％的浓度和16 gL的^-1内5小时 在相同的反应时间内，固体含量增加至25％（m / m）导致葡萄糖产率和浓度分别为75％和70 gL ^-1。