Background

Considering the increasing prevalence of alcohol use and the growing number of orthodontic patients, some orthodontic patients might engage in binge drinking during treatment. Nevertheless, little is known about the effect of alcohol use on orthodontic treatment.

Methods

Male Wistar rats were divided into ethanol and control groups (n = 32). The rats received a single daily intraperitoneal injection of 20% (vol/vol) ethanol/saline solution at a dose of 3 g/kg of ethanol or saline for three consecutive days, and no injection was given during the remaining four days each week. All rats received orthodontic appliances to draw the maxillary first molar mesially. The rats were sacrificed at days 14 and 28, respectively. The amount of tooth movement was measured. Root resorption area was evaluated by scanning electron microscope. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were conducted. Immunohistochemistry staining was performed to evaluate the expressions of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and inducible nitric oxide synthase (iNOS).

Results

There were no significant differences in tooth movement and root resorption between ethanol and control groups. The number of TRAP-positive cells was significantly higher in the ethanol group. The expression of RANKL was statistically increased in the ethanol group. In contrast, the expression of OPG was remarkably decreased in rats injected with ethanol. Moreover, the iNOS level was significantly up-regulated in the ethanol group.

Conclusion

The tooth movement and root resorption in rats were not affected by binge alcohol exposure.

中文翻译：

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暴饮暴食对大鼠牙齿运动和相关牙根吸收的影响。

背景

考虑到酒精使用率的增加和正畸患者数量的增加，一些正畸患者在治疗期间可能会酗酒。然而，关于饮酒对正畸治疗的影响知之甚少。

方法

雄性 Wistar 大鼠分为乙醇组和对照组（n = 32）。大鼠连续3天每天单次腹腔注射20%（vol/vol）乙醇/生理盐水，剂量为3 g/kg乙醇或生理盐水，每周剩余4天不注射。所有大鼠均接受正畸矫治器以向近中绘制上颌第一磨牙。分别在第14天和第28天处死大鼠。测量牙齿移动的量。通过扫描电子显微镜评估牙根吸收面积。进行苏木精和伊红 (H&E) 染色和抗酒石酸酸性磷酸酶 (TRAP) 染色。进行免疫组织化学染色以评估核因子κB配体（RANKL）、骨保护素（OPG）和诱导型一氧化氮合酶（iNOS）的表达。

结果

乙醇组和对照组之间的牙齿移动和牙根吸收没有显着差异。乙醇组中TRAP阳性细胞的数量显着增加。RANKL的表达在乙醇组中有统计学增加。相反，注射乙醇的大鼠中 OPG 的表达显着降低。此外，乙醇组的 iNOS 水平显着上调。

结论

大鼠的牙齿运动和牙根吸收不受暴饮暴食的影响。