Abstract

Methods for phosphorylation of tyrosine and Tyr-Thr-Lys tripeptide with cyclohexylmethyl(deuteromethyl)phosphonochloridates have been developed. These products can be used as reference compounds in the analysis of blood plasma of patients presumably exposed to cyclohexylmethylphosphonofluoridate (cyclosarin) action. Conditions for the separation and purification of the synthesized intermediates by means of chromatography have been determined and optimized, allowing high-purity phosphorylated products at the final stage of the synthesis.

中文翻译：

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酪氨酸和Tyr-Thr-Lys三肽被环己基甲基和（氘代甲基）膦酰氯磷酸化

摘要

已经开发了用环己基甲基（氘代甲基）膦酰氯将酪氨酸和Tyr-Thr-Lys三肽磷酸化的方法。这些产品可用作分析可能暴露于环己基甲基氟膦酸酯（cyclosarin）作用的患者血浆的参考化合物。已经确定并优化了通过色谱法分离和纯化合成中间体的条件，使得在合成的最后阶段可以得到高纯度的磷酸化产物。