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Synthesis of Dihydroquinoline-Based Derivatives of Fluorescent Rhodamine Dyes for Nucleic Acid Analysis by a Real-Time Polymerase Chain Reaction
Russian Journal of Bioorganic Chemistry ( IF 1 ) Pub Date : 2020-05-01 , DOI: 10.1134/s1068162020030127
I. V. Matvienko , V. M. Bayramov , N. A. Parygina , V. E. Kurochkin , Y. I. Alekseev

Abstract An optimized synthesis scheme for the dihydroquinoline-based derivatives of xanthene fluorescent dye has been developed. For the first time, their use as effective fluorophores in hybridization probes for real-time PCR has been demonstrated with the detection on an individual spectral channel.

中文翻译:

通过实时聚合酶链反应合成用于核酸分析的荧光罗丹明染料的二氢喹啉衍生物

摘要 开发了基于二氢喹啉的呫吨荧光染料衍生物的优化合成方案。首次通过单个光谱通道上的检测证明了它们在实时 PCR 杂交探针中作为有效荧光团的用途。
更新日期:2020-05-01
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